摘要
为了研究不同杜仲叶茶的活性成分及抗氧化特性,以杜仲叶为原料,采用不同加工方式将杜仲叶制成绿茶、黑茶、植物乳植杆菌(Lactiplantibacillus plantarum)发酵茶和冠突散囊菌(Eurotium cristatum)发酵茶,对其活性成分及抗氧化活性进行分析,并对两者相关性进行分析.结果表明,杜仲叶绿茶的活性成分含量最高,其总多糖含量、总多酚含量、总黄酮含量、绿原酸含量分别为176.31 mg/g、1.25mg/g、78.77 mg/g、75.51 mg/g;其抗氧化能力最佳,DPPH、ABTS+自由基清除率、铁离子还原/抗氧化能力分别为62.85%、36.04%、34.90.总多糖、绿原酸含量与DPPH自由基清除率显著相关(P<0.05),总黄酮、总多酚含量与DPPH自由基清除率极显著相关(P<0.01).因此,总多酚是杜仲叶茶发挥抗氧化作用的物质基础.
Abstract
In order to study the active components and antioxidant properties of different Eucommia ulmoides leaves tea,the green tea,black tea,Lacti-plantibacillus plantarum fermented tea and Eurotium cristatum fermented tea were prepared from E.ulmoides leaves by different processing methods,the active ingredients and antioxidant activity were analyzed,and the correlation between them was analyzed.The results showed that the active components contents of Eurotium cristatum leaves green tea were the highest,the total polysaccharides,total polyphenols,total flavonoids and chlorogenic acid contents were 176.31 mg/g,1.25 mg/g,78.77 mg/g and 75.51 mg/g,respectively.The antioxidant capacity was the optimal,the DPPH,ABTS+radicals scavenging rates,Ferric ion reducing antioxidant power were 62.85%,36.04%and 34.90,respectively.The contents of total polysaccharides and chloro-genic acid were significantly correlated with DPPH radicals scavenging rates(P<0.05),and the contents of total flavonoids and total polyphenols were extremely significantly correlated with DPPH radicals rates(P<0.01).Therefore,the total polyphenols were the material basis for the antioxidant effect of E.ulmoides leaves tea.