首页|正常和瓮臭红酸汤风味特征、微生物群落差异及相关性分析

正常和瓮臭红酸汤风味特征、微生物群落差异及相关性分析

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该研究采用电子鼻、电子舌、气相色谱-质谱(GC-MS)联用和高通量测序技术检测正常风味(编号为LSN)和瓮臭味(编号为LS42、LS44和LS143)红酸汤感官品质、挥发性风味成分和微生物群落,并采用香气活度值(OAV)分析不良风味的可能来源及其与微生物的相关性。结果表明,瓮臭味红酸汤味觉丰富性较强,但其酸、咸、鲜等口感弱于正常样品。红酸汤共检出挥发性风味物质138种,酯类、烯萜类和醇类化合物的种类和含量占绝对优势,在LSN、LS42和LS143样品中占所有挥发性物质含量的91。19%、85。77%和91。22%。相较而言,瓮臭味红酸汤样品LS143中检测到正戊酸(13。599 μg/g)、丁酸(7。319 μg/g)等具有异臭的短链脂肪酸存在且其OAV>1,风味贡献显著。产黄杆菌属(Rhodanobacter sp。)001617365、耐酸乳杆菌(Lactobacillus acetotolerans)、栖密歇根湖沉积物杆状菌(Sediminibacterium magnilacihabitans)、花生根瘤菌属(Bradyrhizobium sp。)000938305、口乳杆菌(Limosilactobacillus oris)和班图酒香酵母(Brettanomyces custersianus)是正常红酸汤风味的潜在贡献微生物,嗜几丁质菌属(Chitinophaga vietnamensis)、中间根瘤菌属(Mesorhizobium terrae)、产黄杆菌属(Rhodanobacter sp。)001617365与丁酸、戊酸等呈正相关,可能是导致红酸汤出现瓮臭味的主要因素之一。
Difference of flavor characteristics and microbial community of normal and urn odor of red sour soups and correlation analysis
In this study,the sensory quality,volatile flavor components and microbial communities of normal flavor(LSN)and urn odor(LS42,LS44 and LS143)red sour soups were determined using electronic nose,electronic tongue,gas chromatography-mass spectrometry(GC-MS)and high throu-ghput sequencing technology.Possible sources of undesirable flavors and their correlations with microorganisms were analyzed using aroma activity values(OAV).The results showed that the taste richness of the urn odor red sour soup was stronger,but its sour,salty,and fresh flavors were weaker than those in normal samples.A total of 13 8 volatile flavor compounds were detected in the red sour soups,and the types and contents of esters,alkenes and alcohols were absolute dominant,accounting for 91.19%,85.77%and 91.22%of all volatile substances in samples LSN,LS42 and LS143.Compared with the normal samples,the short-chain fatty acids with odor such as n-valeric acid(13.599 μg/g)and butyric acid(7.319 μg/g)were detected in the sample LS143,and their OAV was greater than 1,which contributed significantly to flavor.Rhodobacter sp.001617365,Lactobacillus acetoolerans,Sediminibacterium magnilica habituans,Bradyrhizobium sp.000938305,Limosilactobacillus oris,and Brettanomyces custersianus were potential contributing microorganisms to the normal flavor of red sour soup.Chitinophaga vietnamensis,Mesohizobium terrae,and Rhodobacter sp.001617365 were positively correlated with butyric acid,valeric acid,etc.,which might be one of the main factors causing the urn odor of red sour soup.

red sour soupurn odorflavor characteristicsmicrobial communitycorrelation analysis

何扬波、李国林、石彬、李伟、李咏富、郑汝忠、祝婷、杨一

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贵州省现代农业发展研究所,贵州贵阳 550006

贵州大学农学院,贵州贵阳 550025

贵州省高等学校设施蔬菜工程研究中心,贵州贵阳 550025

安顺市平坝区万佳农产品开发有限公司,贵州安顺 561107

麻江县明洋食品有限公司,贵州麻江 557603

安顺市平坝区齐伯镇农业服务中心,贵州安顺 561100

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红酸汤 瓮臭味 风味特征 微生物群落 相关性分析

2024

中国酿造
中国调味品协会 北京食品科学研究院

中国酿造

CSTPCD北大核心
影响因子:0.759
ISSN:0254-5071
年,卷(期):2024.43(12)