In order to quickly and efficiently detect the contents of β-1,3-glucan and β-1,3-1,6-glucan produced by microorganisms,the reaction con-ditions of Congo red method were optimized by single factor tests and response surface tests,and the methodology was investigated.The results showed that the optimal reaction conditions were Congo red mass concentration 0.2 g/L,reaction temperature 20 ℃,time 35 min,and pH 7.Under the optimized reaction conditions,the absorbance values of β-1,3-glucan and β-1,3-1,6-glucan were 0.645 and 0.734 respectively.The detection limits and quantification limits of the two glucans were 0.1 g/L,2.0 g/L and 0.1 g/L,0.8 g/L,respectively.The relative standard deviations(RSDs)of precision test results of the two β-glucans were 1.09％and 1.15％,the average recovery rates were 99.6％and 100.74％,and the RSD of recovery rates test results were 2.27％and 2.94％,respectively.The results showed that the Congo red method had good precision and accuracy,and was suitable for quantitative analysis of two β-glucans produced by microorganisms.
关键词
β-1,3-葡聚糖/β-1,3-1,6-葡聚糖/刚果红法/检测条件优化/响应面法
Key words
β-1,3-glucan/β-1,3-1,6-glucan/Congo red method/detection conditions optimization/response surface method
维普
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