Determination of 8 sweeteners and ethyl carbamate in Baijiu by ultra performance liquid chromatography-tandem mass spectrometry
A method was established for the determination of ethyl carbamate(EC)and 8 kinds of sweeteners(sucralose,saccharin sodium,cyclamate,acesulfame potassium,aspartame,neotame,alitame,stevioside)in Baijiu by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The Baijiu samples were filtered by 0.22 µm organic filter film and directly injected for mass spectrometry,separated using Agilent ZORBAX RRHD Eclipse Plus C18 column(100 mm×2.1 mm,1.8 µm)with gradient elution of methanol and 0.1％acetic acid aqueous solution,column temperature 40 ℃ and flow rate 0.35 ml/min,and detected by switching between positive and negative ion modes using ULPC-MS/MS.The injection time was 9 min.The results showed that 9 tested substances had a good linear relationship in the range of 2-500 μg/L,the correlation coefficients(R2)were all greater than 0.997,the detection limits were 0.1-0.4 μg/L,and the recoveries were 92.6％-108.7％under the spiked levels of 100 μg/L,and the relative standard deviations(RSD)were lower than 2％.The detection method had the advantages of simple pretreatment,good reproducibility,high sensitivity and fast analysis,and could be used for rapid detection and daily monitoring of EC and 8 kinds of sweeteners in Baijiu.
万方数据
维普
