中国酿造2025,Vol.44Issue(8) :184-190.DOI:10.11882/j.issn.0254-5071.2025.08.027

植物乳植杆菌发酵对南果梨汁品质和挥发性风味成分的影响

Effect of Lactiplantibacillus plantarum fermentation on the quality and volatile flavor components of Nanguo pear juice

王思凯 赵新玉 毛华 张靖楠 郭鹏 张峻松
中国酿造2025,Vol.44Issue(8) :184-190.DOI:10.11882/j.issn.0254-5071.2025.08.027

植物乳植杆菌发酵对南果梨汁品质和挥发性风味成分的影响

Effect of Lactiplantibacillus plantarum fermentation on the quality and volatile flavor components of Nanguo pear juice

王思凯 1赵新玉 2毛华 2张靖楠 1郭鹏 2张峻松1
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作者信息

  • 1. 郑州轻工业大学烟草科学与工程学院,河南郑州 450000
  • 2. 红塔辽宁烟草有限责任公司,辽宁沈阳 110001
  • 折叠

摘要

采用植物乳植杆菌(Lactiplantibacillus plantarum)发酵南果梨汁,研究南果梨汁发酵过程中的理化性质(pH值、总酸、总糖、还原糖、总酚、总黄酮)及抗氧化能力的变化规律,并采用气相色谱-质谱(GC-MS)技术分析发酵前后南果梨汁中挥发性香味物质.结果表明,植物乳植杆菌在南果梨汁中生长良好,发酵36 h时活菌数达到8.89 lg(CFU/mL).南果梨汁发酵过程中,还原糖含量下降了30.57%,pH值下降了 1.59,总酸、总酚和总黄酮含量分别增加了60%,78.65%和79.21%,发酵南果梨汁对1,1-二苯基-2-三硝基苯肼(DPPH)和2,2′-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)自由基清除率显著提高,分别为57.41%和65.06%.GC-MS分析结果显示,植物乳植杆菌发酵南果梨汁前后、未接菌发酵南果梨汁分别检出 64种、72种、55种挥发性风味物质,植物乳植杆菌发酵使南果梨汁挥发性香味成分种类和含量增加,其中醇类、酯类、酮类物质相对含量增加62.22%、6.34%、72.11%.南果梨汁发酵24h时风味最佳,感官评分为91.5分,表明植物乳植杆菌发酵能改善南果梨汁的香气和滋味.

Abstract

The Nanguo pear juice was fermented by Lactiplantibacillus plantarum and the variation patterns of physicochemical properties(pH value,total acid,total sugar,reducing sugar,total phenol,total flavonoids)and antioxidant capacity during the fermentation process of Nanguo pear juice were studied.The volatile flavor substances in the Nanguo pear juice before and after fermentation were analyzed by gas chromatography-mass spec-trometry(GC-MS)technology.The results showed that L.plantarum grew well in Nanguo pear juice,reaching a viable count of 8.89 lg(CFU/ml)after fermentation for 36 h.During the fermentation process of Nanguo pear juice,the reducing sugar content decreased by 30.57%,the pH value de-creased by 1.59,and the contents of total acid,total phenol and total flavonoids increased by 60%,78.65%and 79.21%respectively.The radical scav-enging rates of 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)significantly increased,reaching 57.41%and 65.06%,respectively.The GC-MS analysis results showed that 64,72,and 55 volatile flavor substances were detected in before and after the fermentation of Nanguo pear juice by L.plantarum,and Nanguo pear juice fermented without inoculation,respectively.The fermentation by L.plantarum increased the types and contents of volatile flavor components in Nanguo pear juice.Among them,the relative contents of alcohols,esters and ketones increased by 62.22%,6.34%and 72.11%,respectively.The flavor of Nanguo pear juice was the optimal when it was fermented for 24 h,with a sensory score of 91.5 points.This indicated that the fermentation by L.plantarum could improve the aroma and taste of Nanguo pear juice.

关键词

南果梨汁/植物乳植杆菌/品质/挥发性香气成分/抗氧化活性

Key words

Nanguo pear juice/Lactiplantibacillus plantarum/quality/volatile flavor component/antioxidant activity

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出版年

2025
中国酿造
中国调味品协会 北京食品科学研究院

中国酿造

影响因子:0.759
ISSN:0254-5071
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