Mechanism of Taohong Siwu Decoction Regulating MAPK/NF-κB Signaling Pathway in the Treatment of Psoriasis in Rats
Objective To investigate the interventional mechanism of Taohong Siwu Decoction on mitogen-activated protein kinase(MAPK)/nuclear factor kappa-B(NF-κB)signaling pathway-related factors,proteins and genes expression in rat psoriasis models.Methods Seventy-two SD rats were randomly divided into blank group,model group,low-,medium-and high-dose Taohong Siwu Decoction groups,and positive control group.The rat psoriasis-like skin lesion model was induced by applying 5％imiquimod cream on the back of the rats,and simultaneously the modeling was given drug interventions.The psoriasis area and severity index(PASI)was scored by taking photographs,and the histopathological changes of the skin lesions were observed by HE staining for Baker's score.The levels of interleukin(IL)-1β,IL-4,IL-6,IL-36,and tumor necrosis factor(TNF)-α were measured in the peripheral blood of rats by ELISA.Western blot was used to detect p38 MAPK,phosphorylated(p)-p38 MAPK,extracellular signal-regulated kinase 1/2(ERK1/2),NF-κB inhibitory protein(IκB),NF-κB,and p-NF-κB in rat skin lesion tissues.Real-time fluorescence quantitative polymerase chain reaction(PCR)was used to detect the expression levels of p38 MAPK,ERK1/2,IκB,and NF-κB mRNA in rat skin lesion tissues,and laser confocal detection was used to detect the expression of p38 MAPK and IκB proteins in rat skin lesion tissues.Results Compared with the blank group,the PASI score,Baker score in rat skin lesion tissues and the levels of IL-1 β,IL-4,IL-6,IL-36 and TNF-α in peripheral blood of rats in the model group increased significantly;the expression levels of p38 MAPK,ERK1/2,NF-κB mRNA and protein in rat skin lesion tissues were significantly increased,and the expression levels of p-p38 MAPK and p-NF-κB protein were significantly increased,while the expression levels of IκB mRNA and protein were significantly decreased(P＜0.05).Compared with the model group,the PASI score,Baker score in rat skin lesion tissues and the levels of IL-1β,IL-4,IL-6,IL-36 and TNF-αin peripheral blood of rats in each treatment group decreased significantly;the expression levels of p38 MAPK,ERK1/2,NF-κB mRNA and protein in skin lesion tissues decreased significantly,and the expression levels of p-p38 MAPK and p-NF-κB protein decreased significantly,while the expression levels of IκB mRNA and protein increased significantly(P＜0.05).Conclusion By regulating the MAPK/NF-κB signaling pathway,Taohong Siwu Decoction can significantly decrease the levels of IL-1 β,IL-4,IL-6,IL-36,and TNF-α in the peripheral blood of the rat psoriasis model and the expression of p38 MAPK,p-p38 MAPK,ERK1/2,NF-κB,and p-NF-κB in the skin lesion tissues,increase the expression of IκB in the skin lesion tissues,and reduce the degree of inflammatory cell infiltration and epidermal thickening,thus playing a role in the treatment of psoriasis.
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