Studies on High Efficient System for Microspore Culture Technology of Chinese Cabbage
Taking Chinese cabbage〔Brassica campestris L. ssp. pekinensis(Lour)Olsson〕‘Jihong 82’,some of #534DH and #438 population as testing material,studies the effect of mechanical extraction and artificial extrusion on micro-spore viability and extraction quantity.The result indicates that mechanical extraction can replace the artificial one,get multiple samples during short period of time,and rapidly adjust the concentration of microspores culture.40 suitable buds are collected in 5 mL centrilfuge tubes,3 000 r·min-1 micro-spores gained by 10 s smashing extraction are isolated in 25 mL NLN-13 medium.Thus,micro-spore concentration is insured in suitable culture scope,and successfully induced to embryogenesis.
Chinese cabbageIsolated microspore cultureBeads cell disrupter system
国家科技期刊平台
NSTL
万方数据
维普
