首页|甘蓝无蜡粉亮绿突变体材料LD10遗传规律及分子标记研究

甘蓝无蜡粉亮绿突变体材料LD10遗传规律及分子标记研究

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为了明确结球甘蓝无蜡粉亮绿突变体材料LD10的特征特性及遗传规律,测定了LD10的主要农艺性状和营养物质含量;对LD10和普通材料21-3的叶面超微结构进行了观察比较;以LD10和21-3为亲本构建6世代群体,对LD10无蜡粉亮绿性状遗传规律进行分析。结果表明:无蜡粉亮绿突变体材料LD10叶面蜡粉呈小颗粒状结构,缺失严重,而普通材料21-3叶面蜡粉呈长棒状结构,含量丰富,二者具有明显差异;LD10的无蜡粉亮绿性状由1个隐性基因(cgl-4)控制,其与SSR标记LT-SSR16紧密连锁,遗传距离为4.7 cM。用LD10与另一单基因隐性遗传的无蜡粉亮绿突变体材料10Q-961杂交获得F1群体,F1群体单株全部表现为叶面有蜡粉,推断LD10与10Q-961的无蜡粉亮绿基因位于不同的遗传位点。
Studies on Inheritance and Molecular Marker in Cabbage Glossy Wax-less Mutant LD10
To clarify the characteristics and inheritance of cabbage(Brassica oleracea L. var. capitataL.) glossy wax-less mutant LD10,the major agronomic characteristics and nutrient contents of LD10 were tested, and ultra-structures of leaves in mutant material LD10 and normal material 21-3 were measured,observed and comparatively analyzed. LD10 and 21-3 were taken as parent to construct 6 generations population,and inheritance analysis onthe glossy wax-less characteristics of LD10 was conducted. The results showed that there was little wax on the leaves of LD10,while the wax on the leaves of 21-3 was abundent,and the granular wax in LD10 was of long stick shape,significantly different from the wax in 21-3. The glossy wax-less trait in cabbage LD10 was controlled by one single recessive gene named cgl-4. One molecular marker(LT-SSR16)linked to cgl-4 with genetic distances 4.7 cM. Another F1 population obtained through crossing LD10 and 10Q-961 showed that the genes leading to glossy wax-less trait were at different locations in the two glossy wax-less materials.

CabbageGlossy wax-lessCharacteristicsInheritanceMolecular marker

刘东明、杨丽梅、唐俊、刘泽洲、方智远、刘玉梅、庄木、张扬勇、孙培田、李景涛

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中国农业科学院蔬菜花卉研究所,北京 100081

结球甘蓝 无蜡粉亮绿 特征特性 遗传规律 分子标记

国家大宗蔬菜产业技术体系项目国家“863”计划项目“十二五”国家科技支撑计划项目农业部园艺作物生物学与种质创制重点实验室项目

nycytx-35-gw012012AA1001012012BAD02B01

2014

中国蔬菜
中国农业科学院蔬菜茶卉所

中国蔬菜

CSTPCD北大核心
影响因子:0.545
ISSN:1000-6346
年,卷(期):2014.(12)
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