Studies on Inheritance and Molecular Marker in Cabbage Glossy Wax-less Mutant LD10
To clarify the characteristics and inheritance of cabbage(Brassica oleracea L. var. capitataL.) glossy wax-less mutant LD10,the major agronomic characteristics and nutrient contents of LD10 were tested, and ultra-structures of leaves in mutant material LD10 and normal material 21-3 were measured,observed and comparatively analyzed. LD10 and 21-3 were taken as parent to construct 6 generations population,and inheritance analysis onthe glossy wax-less characteristics of LD10 was conducted. The results showed that there was little wax on the leaves of LD10,while the wax on the leaves of 21-3 was abundent,and the granular wax in LD10 was of long stick shape,significantly different from the wax in 21-3. The glossy wax-less trait in cabbage LD10 was controlled by one single recessive gene named cgl-4. One molecular marker(LT-SSR16)linked to cgl-4 with genetic distances 4.7 cM. Another F1 population obtained through crossing LD10 and 10Q-961 showed that the genes leading to glossy wax-less trait were at different locations in the two glossy wax-less materials.