Taking ‘Jingxin No.1’ watermelon as experimental material,this paper investigated the effects of different humic substances(HS) concentration (0,0.1,0.3,0.6,1.0 g·L-1) in Hoagland on photosynthesis, chlorophyll content,biomass,electrolyte leakage percentage,antioxidant enzyme,proline and Na+,K+ contents of watermelon seedling under salt stress(100 mmol·L-1 NaCl).The results showed that adding HS could improve the plant growth by different degrees under salt stress,especially the 0.3 g·L-1 treatment had the best alleviation effect,in which the chlorophyll contents,photosynthesis,Fv/Fm and ΦPSⅡ were significantly increased,and the distribution of Na+ and K+ was improved. At the same time,the activities of SOD and CAT were enhanced,and the electrolyte leakage percentage and MDA accumulation were reduced. Thus,watermelon plant growth was promoted.