摘要
为探索对西瓜病毒病具有防效作用的植物免疫诱抗剂,以小西葫芦黄花叶病毒(zucchini yellow mosaic virus,ZYMV)为防治对象,植株的显症率及相对防效为指标,评价了6种不同浓度的哌啶酸(Pip)、N-羟基哌啶酸(NHP)、没食子酸、苯并噻二唑(BTH)、壳寡糖、β-氨基丁酸(β-aminobutyric acid,BABA)及其2种药剂和3种药剂复配后对该病毒的防治效果.结果显示,单一药剂喷施后对西瓜ZYMV的防效从高到低依次为:NHP(0.5 mmol·L-1)、Pip(2 mmol·L-1)、壳寡糖(1 g·L-1)、没食子酸(0.5 g·L-1)、BABA(1 mmol·L-1)、BTH(0.1 mmol·L-1);复配药剂试验中,2种药剂复配防效最好的是2-Ⅶ(NHP 0.5 mmol·L-1+Pip 2 mmol·L-1),接种后11 d、无菌水对照植株显症率达到100.00％时,该组合处理的植株显症率为10.00％,相对防效达89.99％,但该复配药剂会影响植株生长;3种药剂复配防效较好的组合是3-Ⅰ(没食子酸 0.5 g·L-1+NHP 0.5 mmol·L-1+BABA 1 mmol·L-1)和3-Ⅱ(没食子酸 0.5 g·L-1+NHP 0.5 mmol·L-1+Pip 2 mmol·L-1),显症率分别为27.27％、33.33％,相对防效分别为79.49％、68.27％,显著高于其他组合,其中3-Ⅱ处理的植株显症率增加缓慢,防病效果较其他处理持久,防治效果最优.综上,NHP、Pip和没食子酸3种诱抗剂在预防西瓜病毒病害中具有潜在的应用价值.
Abstract
In order to explore plant immunoinducer with control effect to watermelon virus disease,taking zucchini yellow mosaic virus(ZYMV)as control object,plant pathogenicity and relative control efficiency as indicators,this paper evaluated 6 agents with different concentrations,including picolinic acid(Pip),N-hydroxy-picolinic acid(NHP),gallic acid,benzothiadiazole(BTH),chitosan,β-aminobutyric acid(BABA),and their two-agent and three-agent combinations for their control effects on this virus disease.The results showed that the control effect of single-agent on ZYMV in watermelon ranked from high to low as follows:NHP(0.5 mmol·L-1),Pip(2 mmol·L-1),chitosan(1 g·L-1),gallic acid(0.5 g·L-1),BABA(1 mmol·L-1),BTH(0.1 mmol·L-1).In the combination agent experiment,the best control effect of the two-agent combination was 2-Ⅶ(NHP 0.5 mmol·L-1+Pip 2 mmol·L-1).Eleven days after inoculation,when incidence rate of the plant by control-treated water reached 100.00％,the incidence rate of the combination-treated plants was 10.00％,and relative control effect was 89.99％.However,this combination agent would affect plant growth.The combination of 3 agents with better control effect were 3-Ⅰ(gallic acid 0.5 g·L-1+NHP 0.5 mmol·L-1+BABA 1 mmol·L-1)and 3-Ⅱ(gallic acid 0.5 g·L-1+NHP 0.5 mmol·L-1+Pip 2 mmol·L-1).Their incidence rates were 27.27％and 33.33％,respectively,and their relative control effects were 79.49％and 68.27％,respectively,significantly higher than those of the other combinations.Among them,the incidence rate of the plants treated with 3-Ⅱ increased slowly,and disease control effect was more lasting than that of the other treatments.Thus,3-Ⅶ had the best control effect on ZYMV.The results indicate that NHP,Pip and gallic acid had potential application value in preventing watermelon virus diseases.
基金项目
国家现代农业产业技术体系项目(CARS-25)
中国农业科学院科技创新工程项目(CAAS-ASTIP-2022-ZFRI-09)
国家自然科学基金项目(31701942)
维普
万方数据