The characteristics of water absorption and retention in substrate can directly affect its moisture content and growth and development of vegetable plug-seedlings.In order to investigate the effect of wetting agent on water absorption and retention in substrate plug-seedling,this paper studied the effects of adding wetting agent on water absorption quantity,and evaporation rate of substrate with different particle sizes,and growth of cucumber plug-seedlings.The results showed that the water absorption rate and absorption quantity of small particle substrate were significantly lower than those of large particle substrate.Wetting agent was more conducive to increase the water absorption quantity of small particle substrate,equivalently to increase 17.32 percentage points comparing with large particle substrate.Adding wetting agent had simultaneously increased the water absorption and evaporation rates of plug-seedling substrate,and maintained a relatively high substrate moisture content throughout the 1,2 and 3 water absorption-evaporation processes.Adding wetting agent to substrate could effectively promote the growth and development of cucumber plug-seedlings under small particle size substrate conditions;and significantly improve the root shoot ratio and clumping rate of cucumber seedlings.The above results indicated that adding wetting agent to substrate could effectively improve the characteristics of water absorption and retention of small particle size nursery substrates,and was conducive for the formation of strong cucumber plug-seedlings.