Molecular Detection of Tomato Spotted Wilt Virus Infected Pepper in Guizhou
Tomato spotted wilt virus(TSWV)was identified by RT-PCR in suspected pepper virus samples collected from 6 regions in Guizhou Province,and the N gene sequences of TSWV in different regions were amplified by molecular cloning.The results showed that TSWV were found in chilies from Guiyang,Anshun,Weng'an,Fuquan,Changshun and Huishui in Guizhou.After sequences aligning,the nucleotide and amino acid identities of the strains isolated from Guizhou were 95.6%-99.7%and 97.1%-100.0%,respectively.Phylogenetic tree analysis suggested that TSWV strains isolated from pepper in Fuquan,Huishui and Changshun were closely related to those in Yunnan and Shandong.The TSWV strains isolated from peppers in Anshun,Guiyang and Weng'an were relatively closed to the strains isolated from tomato,pepper,tobacco,celery and Herb of Spanish needles in Yunnan and lettuce in Jiangsu.The study indicated that there were two TSWV lines in Guizhou pepper,which were certainly correlated with those isolates from various hosts in different regions in China.
peppertomato spotted wilt virusN geneidentification of virusanalysis of sequence
万方数据
维普
