In order to investigate the effect of adding different microbial agents on fungal diversity in strawberry continuous cropping soil after soil disinfection,this experiment set up 7 treatments after dazomet fumigation,including T1(Trichoderma harzianum + Bacillus subtilis + Actinomycetes),T2(Trichoderma harzianum + Bacillus subtilis),T3(Trichoderma harzianum + Actinomycetes),T4(CK),T5(Bacillus subtilis),T6(Actinomycetes),and T7(Trichoderma harzianum);and analyzed the soil fungal community and its composition by high-throughput sequencing platform(Ⅰllumina HiSeq 2500).The results indicated that applying microbial agents could increase fungal abundance,diversity and population quantity.Comparing with T4,single application of microbial agents in T5,T6 and T7 all could increase the species abundance of fungal communities.While,applying mixed microbial agents in T1 and T3 could increase species abundance,but T2 could decrease species abundance.The application of different microbial agents after dazomet fumigation could increase soil fungal diversity.Comparing with the CK,soil fungal populations of other microbial agents were increased by 5.62%-57.54%,except T2,where the fungal population was decreased.In turns from highest to lowest were T6,T7,T3,T1,T5,and T2.After dazomet fumigation,the treatments of microbial agents containing Bacillus subtilis,Actinomycetes,and Trichoderma harzianum could significantly increase soil fungal populations and speed up their recovery rate,especially the populations of beneficial bacteria like Lecanicillium,Cladorrhinum,Remersonia,and Aspergillus.In summary,after dazomet fumigation and disinfection,adding microbial agents to strawberry continuous cropping soil is a scientifically effective method for rebuilding microbial community balance of cultivated soil and alleviating continuous cropping obstacles.
维普
万方数据
