摘要
为研究外源生长调节物质对低温弱光胁迫下辣椒幼苗形态和生理指标的影响,以兴蔬301为试材,采用0.1 μmol·L-1的油菜素内酯(BRs)、100 μmol·L-1 的褪黑素(MT)、500 μmol·L-1 的水杨酸(SA)、100 μmol·L-1 的脱落酸(ABA)、300 mg·L-1 的 6-苄基腺嘌呤(6-BA)、100 μmol·L-1 的茉莉酸甲酯(MeJA)、20 μmol·L-1 的独角金内酯(GR24)7种外源生长调节物质处理辣椒幼苗.结果表明:与常温对照相比,低温弱光胁迫下辣椒幼苗的壮苗指数显著降低,叶片中可溶性糖、脯氨酸和丙二醛含量显著增加;叶绿素含量和过氧化氢酶活性显著下降.与低温弱光对照相比,各外源生长调节物质处理的幼苗壮苗指数和叶片可溶性糖、脯氨酸、叶绿素含量及过氧化氢酶活性明显增加,丙二醛含量降低,叶绿素荧光参数Fv/Fm和光化学猝灭系数(qP)升高,非光化学猝灭系数(NPQ)降低.但不同外源生长调节物质缓解低温弱光胁迫效果有差异,ABA、MT、GR24效果更好.外源生长调节物质处理在低温弱光胁迫前期(5 d)效果明显,恢复生长5 d后差异不显著.表明低温弱光胁迫会影响辣椒幼苗生长,外源生长调节物质处理在早期能有效缓解逆境胁迫对辣椒幼苗的伤害;不同外源生长调节物质处理效果有差异,ABA、MT和GR24能显著增强辣椒幼苗的耐低温弱光能力,其中100 μmol·L-1 的ABA效果最佳.
Abstract
In order to study the effects of exogenous growth regulators on morphological and physiological indexes of seedlings under low-temperature and low-light stress,seven exogenous growth regulators were used to treat chilli seedlings with'Xingshu 301',including 0.1 μmol·L-1 oleandrin lactones(BRs),100 μmol·L-1 melatonin(MT),500 μmol·L-1 salicylic acid(SA),100 μmol·L-1 abscisic acid(ABA),300 mg·L-1 6-benzyl adenine(6-BA),100 μmol·L-1 methyl jasmonate(MeJA),and 20 μmol·L-1 monocrotaline(GR24).The results showed that,compared with the ambient control,the stoutness index of chilli seedlings under low-temperature low-light stress was significantly reduced,and the contents of soluble sugars,proline and malondialdehyde in the leaves were significantly increased;the chlorophyll content and catalase activity were significantly decreased.Compared with the low-temperature and low-light control,the seedling index and leaf soluble sugars,proline,chlorophyll content and catalase activity were significantly increased,malondialdehyde content was decreased,chlorophyll fluorescence parameter Fv/Fm and photochemical burst coefficient(qP)were increased,and non-photochemical burst coefficient(NPQ)was decreased by the treatments of the exogenous growth regulators.However,the effects of different exogenous growth regulators to alleviate low-temperature and low-light stress varied,and ABA,MT,and GR24 were more effective.The effect of exogenous growth regulators was obvious in the early stage of low-temperature and low-light stress(5 days),and the difference was not significant after 5 days of growth resumption.It has shown that low temperature and low light stress would affect the growth of chilli seedlings,and exogenous growth regulator treatments were effective in alleviating the damage of adversity stress on chilli seedlings in the early stage;there were differences in the effects of different exogenous growth regulator treatments,and ABA,MT,and GR24 significantly enhanced the ability of chilli seedlings to tolerate low temperature and low light,of which 100 μmol·L-1 ABA had the best effect.
基金项目
特色蔬菜产业技术体系项目(CARS-24-A-15)
维普
万方数据