Under laboratory condition,this experiment took β-glucan,wheat bran and cell wall of Fusarium oxysporum as the only carbon source to induce Trichoderma harzianum Th-30 fermentation and β-glucanase production,and isolate and purify β-glucanase by ammonium sulfate salting out and agarose gel column chromatography.Taking'Jinyan 4'cucumber as test material,this experiment also explored the effects of β-glucanase on physiological and morphological indexes of resistance,and prevention and control function of cucumber seedling on common soil-borne diseases of cucumber.The results showed that the activities of β-glucanase from Trichoderma Th-30 fermentation induced under different conditions were significantly different.The enzyme activity of crude enzyme liquid induced by β-glucan was the highest,reaching 75.63 U·mL-1 at 72 h of fermentation.The specific activity of purified β-glucanase was 783.56 U·mg-1,being 45.32 times of the crude enzyme.The treatments of 5-fold solution and 10-fold solution of β-glucanase could increase the root activity and free proline content,PAL,POD,and PPO activities of cucumber seedling,as well as the root shoot ratio and healthy seedling strength index,and the control effects of 10-fold solution of β-glucanase on cucumber Rhizoctonia root rot,cucumber Fusarium wilt,cucumber blight,cucumber damping-off,cucumber stem rot were 50.36%-80.63%.
Trichoderma harzianumβ-glucanaseseparation and purificationcucumber seedlingbiocontrol function
国家科技期刊平台
NSTL
维普
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