In order to definite the pathogen of damping offat seeding stage of wax gourd from the Nanning region of Guangxi,and screen effective control fungicides,this study isolated and purified the pathogen from damping off at seeding stage of wax gourd by tissue separation method,screend 14 kinds of fungicides,and evaluated their control effects.A fungus was isolated from damping offat seeding stage of wax gourd,and the strain was named as DGLK1.After pathogenicity tests,morphological observation and molecular identification,it was confirmed that Rhizoctonia solani AG4HG-Ⅰwas the causal of damping offon wax gourd seeding,which is reported for the first time in China.Among 14 fungicides,450 g·L-1 prochloraz EW had the strongest inhibitory effect with EC50 of 0.220 3 mg·L-1,followed by 500 g·L-1 iprodione SC,40%quintozene·carbcndazim WP,430 g·L-1 tebuconazole SC,75%trifloxystrobin·tebuconazole WDG.They could be used as candidate agents for field control on damping offon wax gourd seeding.