To mining the key genes of drought resistant genes in broccoli,the effect of biomass and antioxidant enzyme activity of broccoli seedlings were analyzed,with'Tailv No.3'for material,simulated drought stress by PEG,and differentially expressed genes related to antioxidant enzymes screened by transcriptome sequencing,then expression level analyzed based on real time fluorescence quantitative PCR.The results showed that the aboveground biomass accumulation of broccoli seedlings was significantly inhibited and the growth of underground parts was promoted,as well as the activities of POD,SOD,CAT,APX and GPX increased.Transcriptome sequencing analysis showed that 6 513 genes differentially expressed was detected in broccoli seedlings under drought stress,of which 3 129 genes were up-regulated and 3 384 genes were down-regulated.45 differentially expressed genes related to antioxidant enzymes were screened,then one gene related to POD,SOD,CAT,APX and GPX was screened with the highest up-regulation amplitude by analysis of relative expression quantity,respectively.These genes may be key genes for drought resistance in broccoli,which provides a scientific basis to research drought resistance mechanism.
维普
万方数据
