To explore the role of the StCDFs transcription factor family in potato stress response,bioinformatics methods were employed to analyze the phylogenetic relationship,gene structure,conserved motifs,cis-acting elements,tissue-specific expression,hormone and stress response of StCDFs.Simultaneously,transcriptome data and qRT-PCR were used to analyze the transcription levels of StCDFs after different cold-tolerant varieties were treated with low temperature stress.A total of 5 StCDFs members(StCDF1,StCDF2,StCDF3,StCDF4,StCDF5)were identified in potato.The CDF family of potato is closely related to tomato.The analysis of gene structure and conserved Motif revealed that Motif 1 was the most widely distributed and conserved in the potato StCDF.Cis-acting element analysis showed that the upstream promoter of StCDFs gene contained a wide range of hormone and stress response elements,and stress response elements accounted for the largest proportion.Tissue-specific expression analysis revealed that StCDFs exhibited distinct expression patterns in different tissues.Additionally,most of these genes were found to respond to stress treatment.Based on the RNA-seq data,the StCDFs were found to respond positively to low temperature stress treatment.Subsequently,the qRT-PCR was performed to explore the expression levels of StCDFs and found that the expression levels of StCDF1,StCDF2 and StCDF3 were up-regulated under low temperature stress.
关键词
马铃薯/CDF/转录因子/激素/低温胁迫/lncRNA
Key words
potato/CDF/transcription factors/hormone/low temperature stress/lncRNA
维普
万方数据