The hypocotyl is a commonly used explant for genetic transformation of cabbage(Brassica oleracea var.capitata),however,the problems of genotype-dependent and low regeneration efficiency are the main obstacles at present.This study explores the construction of a genetic transformation system using the epicotyl of cabbage as an explant,with 9 cabbage varieties as test materials.The regeneration efficiency after Agrobacterium infection was compared between the hypocotyl and epicotyl as two explants.The results show that 5 cabbage varieties had elongated epicotyls.After Agrobacterium inoculation,all 5 varieties with epicotyls as explant induced adventitious shoots with regeneration frequencies ranging from 32.4％to 78.4％.In contrast,only 3 varieties induced of adventitious buds with hypocotyl as explant,with the highest regeneration frequency of only 2.7％.This shows that the epicotyl is an ideal explant for overcoming genotypic dependence in B.olerecea transformation,and provides an efficient approach to obtain regenerated B.oleracea plants.
维普
万方数据