山药块茎生长膨大相关基因的筛选与分析

Screening and Analysis of Genes Related to Growth and Expansion of Yam(Dioscorea alata L.)Tubers

梁任繁 ¹黄皓 ²周生茂 ²王爱勤 ³詹洁 ³文俊丽 ²何龙飞³

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作者信息

1. 广西大学农学院,广西南宁 530004;广西农业科学院,广西南宁 530007
2. 广西农业科学院,广西南宁 530007
3. 广西大学农学院,广西南宁 530004
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摘要

为筛选山药块茎生长膨大相关基因,采用生物信息学方法,对山药块茎发育前期和中期构建的不同发育阶段抑制性消减杂交(suppression subtractive hybridization,SSH)文库进行分析.结果表明:山药块茎发育前期与中期差异表达基因(differential expression genes,DEGs)序列主要涉及九大类,其中发育调控占比最大(27.1％),其他依次为细胞组织结构(14.0％)、物质代谢(11.2％)、生物合成(4.7％)等.初步筛选出泛素-连接酶体(F-box associated,F-box)、碳酸酐酶(carbonic anhydrase,CAs)、核苷二磷酸激酶(nucleoside diphosphate kinase,NDPK)、氨基酸透性酶(amino acid permease)、糖基转移酶(glycosyl transferase)、α-淀粉酶(α-amylase,AMY)等候选基因,它们可能与细胞增殖、细胞伸长、激素代谢、酸碱环境调节及功能物质代谢物等功能有关,参与调控山药块茎的生长膨大.

Abstract

To screen the genes related to the growth and expansion of yam tubers,bioinformatics methods were used to analyzed the suppression subtractive hybridization(SSH)libraries at different developmental stages of yam tubers in the early and middle stages.The results showed that the differentially expressed genes(DEGs)sequences in the early and middle stages of the development of yam tubers mainly involved nine categories,of which developmental regulation accounts for the largest proportion(27.1％),followed by cellular tissue structure(14.0％),material metabolism(11.2％),and biosynthesis(4.7％).Preliminary screening has identified some genes,including F-box associated,carbonic anhydrase(CAs),nucleoside diphosphate kinase(NDPK),amino acid permease,glycosyl transferase,and alpha amylase.These genes may be related to cell proliferation,elongation,hormone metabolism,acid-base environment regulation,and functional metabolite regulation,and are involved in regulating tuber growth and expansion in yam.

关键词

山药/块茎/生长膨大/相关基因/筛选和分析

Key words

yam(Dioscorea alata L.)/tuber/growth and expansion/related genes/screening and analysis

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基金项目

国家重点研发计划项目(2023YFD1200201)

财政部和农业农村部:国家现代农业产业技术体系项目(CARS-21)

出版年

2024

中国蔬菜

中国农业科学院蔬菜茶卉所

中国蔬菜

CSTPCD北大核心

影响因子：0.545

ISSN：1000-6346

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