Taking 37 lettuce varieties(lines)with common market types as test materials,this study screened out new and excellent lettuce germplasms by open field,single-span greenhouse and multi-span greenhouse cultivation methods,and through comprehensive identification and evaluation on leaf traits,single plant weight,flavonoid content and other indicators.The results showed that 37 lettuce varieties(lines) were clustered into 5 groups.The single plant weights and flavonoid contents of lettuce cultivated in open fields were generally higher.The genetic diversity of loose-leaf lettuce was rich.The germplasms among loose-leaf lettuce with better performance were'G21K201'W425'and'H20K995'.Those had better promotional value in Shanghai area.