中国蔬菜2024,Issue(12) :98-105.DOI:10.19928/j.cnki.1000-6346.2024.3053

豌豆镰刀菌根腐病病原菌的分离鉴定

Isolation and Identification of Fusarium Root Rot Pathogen in Pea

和国优 张义杰 周敏 吕亚媚 王婧冉 周绍松 李军营 孔垂思
中国蔬菜2024,Issue(12) :98-105.DOI:10.19928/j.cnki.1000-6346.2024.3053

豌豆镰刀菌根腐病病原菌的分离鉴定

Isolation and Identification of Fusarium Root Rot Pathogen in Pea

和国优 1张义杰 1周敏 1吕亚媚 2王婧冉 3周绍松 1李军营 4孔垂思1
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作者信息

  • 1. 云南省农业科学院农业环境与资源研究所,云南昆明 652025
  • 2. 云南省烟草公司红河州公司,云南红河 661400
  • 3. 昆明市晋城街道办事处城市管理综合服务中心(农科),云南昆明 650605
  • 4. 云南省烟草农业科学研究院,云南昆明 650021
  • 折叠

摘要

为明确云南省豌豆镰刀菌根腐病的病原菌种类,从大理州、昆明市、红河州采集豌豆根腐病病样,采用组织分离法分离并纯化病原菌,根据形态学和分子生物学进行菌株鉴定.结果表明,通过组织分离法共获得真菌菌株85个,经鉴定这些菌为尖孢镰刀菌(Fusarium oxysporum)、茄病镰刀菌(F.solani)和木贼镰刀菌(F.equiseti),其分离频率分别为60%、20%和20%.致病力测定结果表明,这些菌株都能引起豌豆发病,明确其为豌豆根腐病的病原物.3种根腐病病原菌的致病性由强及弱依次为茄病镰刀菌、尖孢镰刀菌、木贼镰刀菌.此外,茄病镰刀菌和木贼镰刀菌首次在云南发现能够引起豌豆根腐病.

Abstract

To identify the pathogens responsible for Fusarium root rot in peas from Yunnan province,samples exhibiting Fusarium root rot were collected from Dali,Kunming,and Honghe City.Tissue isolation method was used to isolate and purify pathogens.Strain identification was conducted based on morphological characteristics and molecular biology techniques.The results indicated that a total of 85 fungal strains were obtained through tissue isolation,which were classified as Fusarium oxysporum,Fusarium solani,and Fusarium equiseti,with isolation frequencies of 60%,20%,and 20%,respectively.Virulence tests showed that these Fusarium strains could cause pea root rot disease and were identified as the pathogen of this disease.The pathogenicity of these three root rot pathogens ranked from strongest to weakest was found to be F.solani,F.oxysporum,and F.equiseti.Notably,this study marks the first report of F.solani and F.equiseti causing pea root rot in Yunnan.

关键词

豌豆/根腐病/尖孢镰刀菌/茄病镰刀菌/木贼镰刀菌

Key words

pea/root rot disease/Fusarium oxysporum/Fusarium solani/Fusarium equiseti

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出版年

2024
中国蔬菜
中国农业科学院蔬菜茶卉所

中国蔬菜

CSTPCD北大核心
影响因子:0.545
ISSN:1000-6346
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