Identification and Genetic Mapping of a Thermo-sensitive White Leaf Mutant in Rice
A thermo-sensitive chlorophyll deficient mutant was obtained from the progeny of tissue culture of a japonica rice cultivar Asominori.The mutant was characterized by albino leaves before 3-leaf stage under low temperature (23℃),while showing normal phenotype under high temperature(30℃).The chlorophyll contents of the mutant were significantly lower than those of wild-type before 3-leaf stage under low temperature,which is due to altered chloroplast development under low temperature.Genetic analysis showed that the mutation was controlled by a single recessive gene,which is tentatively designed as cde2 (chlorophyll deficient 2 ).Furthermore,using an F2 population derived from cde2/Peiai 64 and polymorphic markers between cde2 and Peiai 64,the cde2 was finally localized on the centromeric region of chromosome 1 and positioned between SSR markers RM1 1041 and Indel1 ,within the physical distance about 365 .6 kb.In addition, the expression level of genes associated with chlorophyll biosynthesis, photosynthesis and plastidial transcription/translation apparatus was measured and the results shows that the cde2 mutation increased the transcript level of chlorophyll biosynthesis genes and the platidial transcription/translation apparatus related genes,but reduced the expression level of photosynthesis genes,indicating that CDE2 may involve in the chlorophyll biosynthesis and chloroplast development.
国家科技期刊平台
NSTL
万方数据
维普
