Development of HRM-based Functional Marker for Gelatinization Temperature Gene ALK in Rice
[Objective]Gelatinization temperature is an important parameter in rice eating quality which is mainly controlled by the ALK gene.Fast and high-throughput gene functional markers for ALK can improve selection efficiency in quality improvement.[Method]According to single nucleotide polymorphism in the functional region of ALK alleles,we developed the gene functional marker based on high resolution melting(HRM)technology.[Result]Through PCR amplification and sequencing test,we selected gene markers ALKH4 and ALKH5 in two functional regions of ALK.ALKH4 and ALKH5 were employed to genotype 81 indica varieties and 279 japonica varieties.The results showed that only 16 japonica varieties and 19 indica varieties carrying G-GC genotype were identified as well as 51 japonica varieties carrying A-GC genotype;while 212 japonica varieties and 62 indica varieties fell into G-TT genotype.[Conclusion]The HRM-based functional markers ALKH4 and ALKH5 could distinguish different genotypes of gelatinization temperature ALK locus in a fast and high-throughput manner,and have significance in breeding practice.
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