A β-Carotene Ketolase Gene NfcrtO from Subaerial Cyanobacteria Confers Drought Tolerance in Rice

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外文摘要：Nostoc flagelliforme is a terrestrial cyanobacterium that can resist many types of stressors,including drought,ultraviolet radiation,and extreme temperatures.In this study,we identified the drought tolerance gene NfcrtO,which encodes a β-carotene ketolase,through screening the transcriptome of N.flagelliforme under water loss stress.Prokaryotic expression of NfcrtO under 0.6 mol/L sorbitol or under 0.3 mol/L NaCl stress significantly increased the growth rate of Escherichia coli.When NfcrtO was heterologously expressed in rice,the seedling height and root length of NfcrtO-overexpressing rice plants were significantly higher than those of the wild type(WT)plants grown on ½ Murashige and Skoog solid medium with 120 mmol/L mannitol at the seedling stage.Transcriptome analysis revealed that NfcrtO was involved in osmotic stress,antioxidant,and other stress-related pathways.Additionally,the survival rate of the NfcrtO-overexpression lines was significantly higher than that of the WT line under both hydroponic stress(24%PEG and 100 mmol/L H2O2)and soil drought treatment at the seedling stage.Physiological traits,including the activity levels of superoxide dismutase,peroxidase,catalase,total antioxidant capacity,and the contents of proline,trehalose,and soluble sugar,were significantly improved in the NfcrtO-overexpression lines relative to those in the WT line under 20%PEG treatment.Furthermore,when water was withheld at the booting stage,the grain yield per plant of NfcrtO-overexpression lines was significantly higher than that of the WT line.Yeast two-hybrid analysis identified interactions between NfcrtO and Dna J protein,E3 ubiquitin-protein ligase,and pyrophosphate-energized vacuolar membrane proton pump.Thus,heterologous expression of NfcrtO in rice could significantly improve the tolerance of rice to osmotic stress,potentially facilitating the development of new rice varieties.

外文关键词：

antioxidant enzymeβ-carotene ketolasedrought resistanceNostoc flagelliformeosmotic stressricetranscriptome analysis

作者：

GAO Ningning、YE Shuifeng、ZHANG Yu、ZHOU Liguo、MA Xiaosong、YU Hanxi、LI Tianfei、HAN Jing、LIU Zaochang、LUO Lijun

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作者单位：

College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China

Shanghai Agrobiological Gene Center,Shanghai 201106,China

College of Life Sciences,Shangrao Normal University,Shangrao 334001,China

Institute for Agri-food Standards and Testing Technology,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China

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基金：

National Key Research and Development Program of ChinaScience and Technology Research Project of Jiangxi Provincial Department of Education,ChinaScience and Technology Research Project of Shangrao,Jiangxi Province,China

项目编号：

2018YFE0106200K4100131K4000019

出版年：

2024

DOI：

10.1016/j.rsci.2023.10.002

水稻科学(英文版)

中国水稻研究所

水稻科学(英文版)

CSTPCD

影响因子：0.269

ISSN：1672-6308

年,卷(期)：2024.31(1)

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