Carbon Catabolite Repressor UvCreA is Required for Development and Pathogenicity in Ustilaginoidea virens

XIE Shuwei ¹SHI Huanbin ¹WEN Hui ¹LIU Zhiquan ¹QIU Jiehua ¹JIANG Nan ¹KOU Yanjun¹

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作者信息

1. State Key Laboratory of Rice Biology and Breeding,China National Rice Research Institute,Hangzhou 311400,China
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Abstract

The rice false smut disease,caused by Ustilaginoidea virens,has emerged as a significant global threat to rice production.The mechanism of carbon catabolite repression plays a crucial role in the efficient utilization of carbon nutrients and enzyme regulation in the presence of complex nutritional conditions.Although significant progress has been made in understanding carbon catabolite repression in fungi such as Aspergillus nidulans and Magnaporthe oryzae,its role in U.virens remains unclear.To address this knowledge gap,we identified UvCreA,a pivotal component of carbon catabolite repression,in U.virens.Our investigation revealed that UvCreA localized to the nucleus.Deletion of UvCreA resulted in decreased growth and pathogenicity in U.virens.Through RNA-seq analysis,it was found that the knockout of UvCreA led to the up-regulation of 514 genes and down-regulation of 640 genes.Moreover,UvCreA was found to be involved in the transcriptional regulation of pathogenic genes and genes associated with carbon metabolism in U.virens.In summary,our findings indicated that UvCreA is important in fungal development,virulence,and the utilization of carbon sources through transcriptional regulation,thus making it a critical element of carbon catabolite repression.

Key words

Ustilaginoidea virens/virulence/carbon catabolite repression/Oryza sativa/rice false smut

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基金项目

Key Projects of Zhejiang Provincial Natural Science Foundation,China(LZ23C130002)

国家自然科学基金(32100161)

Zhejiang Science and Technology Major Program on Rice New Variety Breeding,China(2021C02063)

Key Research and Development Project of China National Rice Research Institute(CNRRI-2020-04)

Chinese Academy of Agricultural Sciences under the Agricultural Sciences and Technologies Innovation Program()

Youth innovation Program of Chinese Academy of Agricultural Sciences(Y2023QC22)

Joint Open Competitive Project of the Yazhou Bay Seed Laboratory and China National Seed Company Limited(B23YQ1514)

Joint Open Competitive Project of the Yazhou Bay Seed Laboratory and China National Seed Company Limited(B23CQ15EP)

出版年

2024

水稻科学(英文版)

中国水稻研究所

水稻科学(英文版)

CSTPCD

影响因子：0.269

ISSN：1672-6308

参考文献量51

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