首页|Analysis of RNA Recognition and Binding Characteristics of OsCPPR1 Protein in Rice

Analysis of RNA Recognition and Binding Characteristics of OsCPPR1 Protein in Rice

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Pentatricopeptide repeat(PPR)proteins represent one of the largest protein families in plants and typically localize to organelles like mitochondria and chloroplasts.By contrast,CYTOPLASM-LOCALIZED PPR1(OsCPPR1)is a cytoplasm-localized PPR protein that can degrade OsGOLDEN-LIKE1(OsGLK1)mRNA in the tapetum of rice anther.However,the mechanism,by which OsCPPR1 recognizes and binds to OsGLK1 transcripts,remains unknown.Through protein structure prediction and macromolecular docking experiments,we observed that distinct PPR motif structures of OsCPPR1 exhibited varying binding efficiencies to OsGLK1 RNA.Moreover,RNA-electrophoretic mobility shift assay experiment demonstrated that the recombinant OsCPPR1 can directly recognize and bind to OsGLK1 mRNA in vitro.This further confirmed that the mutations in the conserved amino acids in each PPR motif resulted in loss of activity,while truncation of OsCPPR1 decreased its binding efficiency.These findings collectively suggest that it may require some co-factors to assist in cleavage,a facet that warrants further exploration in subsequent studies.

OsCPPR1RNA recognition and bindingpentatricopeptide repeatrice

ZHENG Shaoyan、CHEN Junyu、Li Huatian、LIU Zhenlan、LI Jing、ZHUANG Chuxiong

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State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Guangdong Laboratory for Lingnan Modern Agriculture/College of Life Sciences,South China Agricultural University,Guangzhou 510642,China

Open Competition Program of Top Ten Critical Priorities of Agricultural Science and Technology Innovation for the 14th Five-国家自然科学基金国家自然科学基金国家自然科学基金Major Program of Guangdong Basic and Applied Research,ChinaDouble First-Class Discipline Promotion Project,China

2022SDZG053200045731871231319210042019B0303020062021B10564001

2024

10.1016/j.rsci.2023.11.011

水稻科学(英文版)
中国水稻研究所

水稻科学(英文版)

CSTPCD
影响因子：0.269
ISSN：1672-6308
年,卷(期)：2024.31(2)
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