Abstract
Prezygotic isolation is important for successful fertilization in rice,significantly affecting yield.This study focused on F5∶6 generation plants derived from inter-subspecific crosses(Nipponbare × KDML105)with low(LS)and high seed-setting rates(HS),in which normal pollen fertility was observed.However,LS plants showed a reduced number of pollen grains adhering to the stigma and fewer pollen tubes reaching the ovules at 4-5 h post-pollination,compared with HS plants.Bulked segregant RNA-Seq analysis of pollinated pistils from the HS and LS groups revealed 249 and 473 differentially expressed genes(DEGs),respectively.Kyoto Encyclopedia of Genes and Genomes analysis of the HS and LS-specific DEGs indicated enrichment in metabolic pathways,pentose and glucuronate interconversions,and flavonoid biosynthesis.Several of these DEGs exhibited co-expression with pollen development genes and formed extensive clusters of co-expression networks.Compared with LS pistils,enzyme genes controlling pectin degradation,such as OsPME35 and OsPLL9,showed similar expression patterns,with higher levels in HS pistils pre-pollination.Os02g0467600,similar to cinnamate 4-hydroxylase gene(CYP73),involved in flavonoid biosynthesis,displayed higher expression in HS pistils post-pollination.Our findings suggest that OsPME35,OsPLL9,and Os02g0467600 contribute to prezygotic isolation by potentially modifying the stigma cell wall(OsPME35 and OsPLL9)and controlling later processes such as pollen-stigma adhesion(Os02g0467600)genes.Furthermore,several DEGs specific to HS and LS were co-localized with QTLs and functional genes associated with spikelet fertility.These findings provide valuable insights for further research on rice spikelet fertility,ultimately contributing to the development of high-yielding rice varieties.
基金项目
Agricultural Research Development Agency of Thailand(PRP6405030280)
Research Promotion fund for International and Educational Excellence,Thailand(08/2562)
维普
万方数据