Appropriate Supply of Ammonium Nitrogen and Ammonium Nitrate Reduces Cadmium Content in Rice Seedlings by Inhibiting Cadmium Uptake and Transport

HU Yunchao ¹YAN Tiancai ¹GAO Zhenyu ¹WANG Tiankang ²LU Xueli ¹YANG Long ¹SHEN Lan ¹ZHANG Qiang ¹HU Jiang ¹REN Deyong ¹ZHANG Guangheng ¹ZHU Li ¹LI Li ²ZENG Dali ³QIAN Qian ¹LI Qing¹

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作者信息

1. State Key Laboratory for Rice Biology and Breeding,China National Rice Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou 311401,China
2. State Key Laboratory of Hybrid Rice,Hunan Hybrid Rice Research Center,Hunan Academy of Agricultural Sciences,Changsha 410125,China
3. State Key Laboratory for Rice Biology and Breeding,China National Rice Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou 311401,China;The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province,College of Advanced Agricultural Sciences,Zhejiang A&F University,Hangzhou 311300,China
折叠

Abstract

Reasonable nitrogen(N)application is a promising strategy for reducing crop cadmium(Cd)toxicity.However,the specific form of N and the required amount that affect Cd tolerance and accumulation in rice remain unclear.This study explored the influence of different N-fertilizer forms(NH4NO3,NH4Cl,and KNO3)and dosages on Cd tolerance and uptake in Cd-stressed N-sensitive and N-insensitive indica rice accessions.The results indicated that the Cd tolerance of N-sensitive indica accessions is more robust than that of N-insensitive ones.Furthermore,the shoot Cd content and Cd translocation rate in both N-sensitive and N-insensitive indica accessions decreased with an appropriate supply of NH4NO3 and NH4Cl,whereas they were comparable or slightly increased with increased KNO3.Unfortunately,we did not find significant and regular differences in Cd accumulation or translocation between N-sensitive and N-insensitive rice accessions.Consistent with the reduction of shoot Cd content,the addition of NH4NO3 and NH4Cl also inhibited the instantaneous root Cd2+uptake.The expression changes of Cd transport-related genes under different N forms and dosages suggested that the decreased shoot Cd content,caused by the increased supply of NH4NO3 and NH4Cl,is likely achieved by reducing the transcription of OsNRAMP1 and OsIRT1.In summary,our findings reveal that an appropriate supply of NH4NO3 and NH4Cl could reduce Cd uptake and transport in rice seedlings,suggesting that rational N management could reduce the Cd risk in rice production.

Key words

Oryza sativa/cadmium/nitrogen/ammonium nitrate/ammonium chloride

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基金项目

National Natural Science Foundation of China(31971872)

Open Research Fund of State Key Laboratory of Hybrid Rice,China(2022KF02)

National Natural Science Foundation of China(32101755)

National Natural Science Foundation of China(32188102)

Zhejiang Provincial Natural Science Foundation,China(LY22C130005)

Key Research and Development Program of Zhejiang Province,China(2021C02056)

the'Pioneer'and'Leading Goose'R&D Program of Zhejiang,China(2023C02014)

出版年

2024

水稻科学(英文版)

中国水稻研究所

水稻科学(英文版)

CSTPCD

影响因子：0.269

ISSN：1672-6308

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