Contextual Fear Learning and Extinction in the Primary Visual Cortex of Mice

Xiaoke Xie ¹Shangyue Gong ²Ning Sun ³Jiazhu Zhu ⁴Xiaobin Xu ³Yongxian Xu ³Xiaojing Li ³Zhenhong Du ⁴Xuanting Liu ³Jianmin Zhang ²Wei Gong ⁵Ke Si⁶

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作者信息

1. Department of Psychiatry,The First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310012,China;College of Biomedical Engineering and Instrument Science,Zhejiang University,Hangzhou 310012,China;Intelligent Optics & Photonics Research Center,Jiaxing Research Institute,Zhejiang University,Jiaxing 314001,China
2. Department of Neurosurgery of the Second Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310012,China
3. MOE Frontier Science Center for Brain Science & Brain-Machine Integration,NHC and CAMS Key Laboratory of Medical Neurobiology,School of Brain Science and Brain Medicine,Zhejiang University,Hangzhou 310012,China
4. Intelligent Optics & Photonics Research Center,Jiaxing Research Institute,Zhejiang University,Jiaxing 314001,China;State Key Laboratory of Modern Optical Instrumentation.College of Optical Science and Engineering,Zhejiang University,Hangzhou 310012,China
5. Department of Neurosurgery of the Second Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310012,China;MOE Frontier Science Center for Brain Science & Brain-Machine Integration,NHC and CAMS Key Laboratory of Medical Neurobiology,School of Brain Science and Brain Medicine,Zhejiang University,Hangzhou 310012,China
6. Department of Psychiatry,The First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310012,China;College of Biomedical Engineering and Instrument Science,Zhejiang University,Hangzhou 310012,China;Intelligent Optics & Photonics Research Center,Jiaxing Research Institute,Zhejiang University,Jiaxing 314001,China;MOE Frontier Science Center for Brain Science & Brain-Machine Integration,NHC and CAMS Key Laboratory of Medical Neurobiology,School of Brain Science and Brain Medicine,Zhejiang Univ
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Abstract

Fear memory contextualization is critical for selecting adaptive behavior to survive.Contextual fear con-ditioning(CFC)is a classical model for elucidating related underlying neuronal circuits.The primary visual cortex(V1)is the primary cortical region for contextual visual inputs,but its role in CFC is poorly understood.Here,our experi-ments demonstrated that bilateral inactivation of V1 in mice impaired CFC retrieval,and both CFC learning and extinc-tion increased the turnover rate of axonal boutons in V1.The frequency of neuronal Ca2+activity decreased after CFC learning,while CFC extinction reversed the decrease and raised it to the naïve level.Contrary to control mice,the frequency of neuronal Ca2+activity increased after CFC learning in microglia-depleted mice and was maintained after CFC extinction,indicating that microglial depletion alters CFC learning and the frequency response pattern of extinction-induced Ca2+activity.These findings reveal a critical role of microglia in neocortical information pro-cessing in V1,and suggest potential approaches for cellular-based manipulation of acquired fear memory.

Key words

Contextual fear conditioning/Calcium imaging/Primary visual cortex/Cortical plasticity/Microglial depletion/Learning and memory

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基金项目

National Natural Science Foundation of China(61735016)

Natural Science Foundation of Zhejiang Province(LR20F050002)

Key R&D Program of Zhejiang Province(2020C03009)

Key R&D Program of Zhejiang Province(2021C03001)

Zhejiang Leading Innovation and Entrepreneurship Team(202099144)

CAMS Innovation Fund for Medical Sciences(2019-I2M-5-057)

Fundamental Research Funds for the Central Universities()

出版年

2023

神经科学通报(英文版)

中国科学院上海生命科学研究院

神经科学通报(英文版)

CSTPCDCSCD

影响因子：0.741

ISSN：1673-7067

参考文献量50

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