Somatostatin-Positive Neurons in the Rostral Zona Incerta Modulate Innate Fear-Induced Defensive Response in Mice

Shan Lin ¹Meng-Yue Zhu ¹Meng-Yu Tang ¹Mi Wang ¹Xiao-Dan Yu ¹Yi Zhu ¹Shi-Ze Xie ¹Dan Yang ²Jiadong Chen ¹Xiao-Ming Li³

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作者信息

1. Department of Neurobiology and Department of Neurology of the Second Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310058,China;NHC and CAMS Key Laboratory of Medical Neurobiology,MOE Frontier Science Center for Brain Research and Brain-Machine Integration,School of Brain Science and Brian Medicine,Zhejiang University,Hangzhou 310058,China
2. Department of Neurobiology and Department of Neurology of the Second Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310058,China
3. Department of Neurobiology and Department of Neurology of the Second Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310058,China;NHC and CAMS Key Laboratory of Medical Neurobiology,MOE Frontier Science Center for Brain Research and Brain-Machine Integration,School of Brain Science and Brian Medicine,Zhejiang University,Hangzhou 310058,China;Center for Brain Science and Brain-Inspired Intelligence,Research Units for Emotion and Emotion Disorders,Chinese Academy of Medical Sciences,China/
折叠

Abstract

Defensive behaviors induced by innate fear or Pavlovian fear conditioning are crucial for animals to avoid threats and ensure survival.The zona incerta(ZI)has been demonstrated to play important roles in fear learning and fear memory,as well as modulating auditory-induced innate defensive behavior.However,whether the neuronal subtypes in the ZI and specific circuits can mediate the innate fear response is largely unknown.Here,we found that somato-statin(SST)-positive neurons in the rostral ZI of mice were activated by a visual innate fear stimulus.Optogenetic inhi-bition of SST-positive neurons in the rostral ZI resulted in reduced flight responses to an overhead looming stimulus.Optogenetic activation of SST-positive neurons in the rostral ZI induced fear-like defensive behavior including increased immobility and bradycardia.In addition,we demonstrated that manipulation of the GABAergic projections from SST-positive neurons in the rostral ZI to the downstream nucleus reuniens(Re)mediated fear-like defensive behavior.Ret-rograde trans-synaptic tracing also revealed looming stim-ulus-activated neurons in the superior colliculus(SC)that projected to the Re-projecting SST-positive neurons in the rostral ZI(SC-ZIrSST-Re pathway).Together,our study elu-cidates the function of SST-positive neurons in the rostral ZI and the SC-ZIrSST-Re tri-synaptic circuit in mediating the innate fear response.

Key words

Innate fear/Zona incerta/Somatostatin-positive neurons/Looming stimulus/Defensive behavior

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基金项目

Research Assistant Shuangshuang Liu from the Core Facilities of Zhejiang University School of Medicine()

Core Facilities of Zhejiang University Institute of Neuroscience()

Science and Technology Innovation 2030—Major Project of Brain Science and Brainlike Research(2021ZD0202700)

Science and Technology Innovation 2030—Major Project of Brain Science and Brainlike Research(2021ZD0202702)

Key-Area Research and Development Program of Guangdong Province(2019B030335001)

Key-Area Research and Development Program of Guangdong Province(2018B030334001)

Major Program of the National Natural Science Foundation of China(82090030)

Major Program of the National Natural Science Foundation of China(82090031)

CAMS Innovation Fund for Medical Sciences(2019-I2M-5-057)

Ministry of Science and Technology(2019YFA0110103)

National Natural Science Foundation of China(81870898)

Fundamental Research Funds for the Central Universities(2021FZZX001-37)

Zhejiang Provincial Natural Science Foundation(LR18H090002)

Young Scientist Program of the National Natural Science Foundation of China(82001135)

出版年

2023

神经科学通报(英文版)

中国科学院上海生命科学研究院

神经科学通报(英文版)

CSTPCDCSCD

影响因子：0.741

ISSN：1673-7067

被引量1

参考文献量44

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