Abstract
Mutations in genes encoding amyloid precursor protein(APP)and presenilins(PSs)cause familial forms of Alzheimer's disease(AD),a neurodegenerative disor-der strongly associated with aging.It is currently unknown whether and how AD risks affect early brain development,and to what extent subtle synaptic pathology may occur prior to overt hallmark AD pathology.Transgenic mutant APP/PS1 over-expression mouse lines are key tools for studying the molecular mechanisms of AD pathogenesis.Among these lines,the 5XFAD mice rapidly develop key features of AD pathology and have proven utility in study-ing amyloid plaque formation and amyloid β(Aβ)-induced neurodegeneration.We reasoned that transgenic mutant APP/PS1 over-expression in 5XFAD mice may lead to neurodevelopmental defects in early cortical neurons,and performed detailed synaptic physiological characterization of layer 5(L5)neurons from the prefrontal cortex(PFC)of 5XFAD and wild-type littermate controls.L5 PFC neurons from 5XFAD mice show early APP/Aβ immunolabeling.Whole-cell patch-clamp recording at an early post-weaning age(P22-30)revealed functional impairments;although 5XFAD PFC-L5 neurons exhibited similar membrane prop-erties,they were intrinsically less excitable.In addition,these neurons received smaller amplitude and frequency of miniature excitatory synaptic inputs.These functional disturbances were further corroborated by decreased den-dritic spine density and spine head volumes that indicated impaired synapse maturation.Slice biotinylation followed by Western blot analysis of PFC-L5 tissue revealed that 5XFAD mice showed reduced synaptic AMPA receptor sub-unit GluA1 and decreased synaptic NMDA receptor subu-nit GluN2A.Consistent with this,patch-clamp recording of the evoked L23＞L5 synaptic responses revealed a reduced AMPA/NMDA receptor current ratio,and an increased level of AMPAR-lacking silent synapses.These results suggest that transgenic mutant forms of APP/PS1 overexpression in 5XFAD mice leads to early developmental defects of corti-cal circuits,which could contribute to the age-dependent synaptic pathology and neurodegeneration later in life.
基金项目
institutional startup funding from the University of Arizona(to SQ)()
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