Abstract
The axon initial segment(AIS)is a highly spe-cialized axonal compartment where the action potential is initiated.The heterogeneity of AISs has been suggested to occur between interneurons and pyramidal neurons(PyNs),which likely contributes to their unique spiking properties.However,whether the various characteristics of AISs can be linked to specific PyN subtypes remains unknown.Here,we report that in the prelimbic cortex(PL)of the mouse,two types of PyNs with axon projections either to the contralat-eral PL or to the ipsilateral basal lateral amygdala,possess distinct AIS properties reflected by morphology,ion chan-nel expression,action potential initiation,and axo-axonic synaptic inputs from chandelier cells.Furthermore,projec-tion-specific AIS diversity is more prominent in the superfi-cial layer than in the deep layer.Thus,our study reveals the cortical layer-and axon projection-specific heterogeneity of PyN AISs,which may endow the spiking of various PyN types with exquisite modulation.
基金项目
National Natural Science Foundation of China(82071450)
National Natural Science Foundation of China(31972903)
National Natural Science Foundation of China(32000681)
Shanghai Pujiang Program(20PJ1401000)
Natural Science Foundation of Shanghai(20ZR1405700)
Shanghai Municipal Science and Technology Major Project(2018SHZDZX01)
ZJ Lab()
Shanghai Center for Brain Science and Brain-Inspired Technology()
Fudan Undergraduate Research Opportunities Program(Wangdao 20046)
维普
万方数据