Abstract
Cortical interneurons can be categorized into distinct populations based on multiple modalities,including molecular signatures and morpho-electrical(M/E)proper-ties.Recently,many transcriptomic signatures based on sin-gle-cell RNA-seq have been identified in cortical interneu-rons.However,whether different interneuron populations defined by transcriptomic signature expressions correspond to distinct M/E subtypes is still unknown.Here,we applied the Patch-PCR approach to simultaneously obtain the M/E properties and messenger RNA(mRNA)expression of＞600 interneurons in layer V of the mouse somatosensory cortex(S1).Subsequently,we identified 11 M/E subtypes,9 neurochemical cell populations(NCs),and 20 transcrip-tomic cell populations(TCs)in this cortical lamina.Further analysis revealed that cells in many NCs and TCs comprised several M/E types and were difficult to clearly distinguish morpho-electrically.A similar analysis of layer V interneu-rons of mouse primary visual cortex(V1)and motor cortex(M1)gave results largely comparable to S1.Comparison between S1,V1,and M1 suggested that,compared to V1,S1 interneurons were morpho-electrically more similar to M1.Our study reveals the presence of substantial M/E varia-tions in cortical interneuron populations defined by molecu-lar expression.
基金项目
National Key Research and Development Program of China(2021ZD0202500)
National Natural Science Foundation of China(31930044)
National Natural Science Foundation of China(31725012)
Foundation of Shanghai Municipal Education Commission(2019-01-07-00-07-E00062)
Collaborative Innovation Program of Shanghai Municipal Health Commission(2020CXJQ01)
Shanghai Municipal Science and Technology Major Project(2018SHZDZX01)
ZJLab()
维普
万方数据