Microglial Depletion does not Affect the Laterality of Mechanical Allodynia in Mice

Quan Ma ¹Dongmei Su ²Jiantao Huo ²Guangjuan Yin ²Dong Dong ²Kaifang Duan ²Hong Cheng ²Huiling Xu ³Jiao Ma ³Dong Liu ²Bin Mou ⁴Jiyun Peng ⁴Longzhen Cheng⁵

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作者信息

1. School of Life Science and Technology,Harbin Institute of Technology,Harbin 150001,China;Shenzhen Key Laboratory of Gene Regulation and Systems Biology,School of Life Sciences,Southern University of Science and Technology,Shenzhen 518055,China;Department of Biology,Brain Research Center,Southern University of Science and Technology,Shenzhen 518055,China
2. Shenzhen Key Laboratory of Gene Regulation and Systems Biology,School of Life Sciences,Southern University of Science and Technology,Shenzhen 518055,China;Department of Biology,Brain Research Center,Southern University of Science and Technology,Shenzhen 518055,China
3. Department of Biology,Brain Research Center,Southern University of Science and Technology,Shenzhen 518055,China
4. Institute of Life Science,Nanchang University,Nanchang 330031,China
5. Shenzhen Key Laboratory of Gene Regulation and Systems Biology,School of Life Sciences,Southern University of Science and Technology,Shenzhen 518055,China;Department of Biology,Brain Research Center,Southern University of Science and Technology,Shenzhen 518055,China;Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions,Shenzhen 518055,China
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Abstract

Mechanical allodynia(MA),including punctate and dynamic forms,is a common and debilitating symptom suffered by millions of chronic pain patients.Some periph-eral injuries result in the development of bilateral MA,while most injuries usually led to unilateral MA.To date,the con-trol of such laterality remains poorly understood.Here,to study the role of microglia in the control of MA laterality,we used genetic strategies to deplete microglia and tested both dynamic and punctate forms of MA in mice.Surpris-ingly,the depletion of central microglia did not prevent the induction of bilateral dynamic and punctate MA.Moreo-ver,in dorsal root ganglion-dorsal root-sagittal spinal cord slice preparations we recorded the low-threshold Aβ-fiber stimulation-evoked inputs and outputs of superficial dorsal horn neurons.Consistent with behavioral results,microglial depletion did not prevent the opening of bilateral gates for Aβ pathways in the superficial dorsal horn.This study chal-lenges the role of microglia in the control of MA lateral-ity in mice.Future studies are needed to further understand whether the role of microglia in the control of MA laterality is etiology-or species-specific.

Key words

Microglia/Mechanical allodynia/Laterality/Spinal dorsal horn/Aβ/Dynamic allodynia/Punctate allodynia

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基金项目

Cheng laboratory for their support and discussions()

Ministry of Science and Technology of China(2021ZD0203302)

National Natural Science Foundation of China(32170996)

National Natural Science Foundation of China(32060199)

Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions(2021SHIBS0002)

Guangdong Science and Technology Committee(2019A1515010041)

Guangdong Science and Technology Committee(A2021319)

Shenzhen Innovation Committee of Science and Technology(ZDSYS20200811144002008)

Shenzhen Science and Technology Innovation Committee(JCYJ20180302174233348)

出版年

2023

神经科学通报(英文版)

中国科学院上海生命科学研究院

神经科学通报(英文版)

CSTPCDCSCD

影响因子：0.741

ISSN：1673-7067

参考文献量77

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