Abstract
The cochlear auditory epithelium contains two types of sound receptors,inner hair cells(IHCs)and outer hair cells(OHCs).Mouse models for labelling juvenile and adult IHCs or OHCs exist;however,labelling for embryonic and perinatal IHCs or OHCs are lacking.Here,we gener-ated a new knock-in Fgf8P2A-3×GFP/+(Fgf8GFP/+)strain,in which the expression of a series of three GFP fragments is controlled by endogenous Fgf8 cis-regulatory elements.After confirming that GFP expression accurately reflects the expression of Fgf8,we successfully obtained both embry-onic and neonatal IHCs with high purity,highlighting the power of Fgf8GFP/+.Furthermore,our fate-mapping analy-sis revealed,unexpectedly,that IHCs are also derived from inner ear progenitors expressing Insm1,which is currently regarded as an OHC marker.Thus,besides serving as a highly favorable tool for sorting early IHCs,Fgf8GFP/+ will facilitate the isolation of pure early OHCs by excluding IHCs from the entire hair cell pool.
基金项目
National Key R&D Program of China(2021YFA1101804)
Strategic Priority Research Program of the Chinese Academy of Science(XDB32060100)
National Natural Science Foundation of China(91949119)
National Natural Science Foundation of China(82101212)
National Natural Science Foundation of China(82101209)
Shanghai Municipal Science and Technology Major Project(2018SHZDZX05)
Science and Technology Commission of Shanghai Municipality(21ZR1440200)
Shanghai Sailing Program(20YF1426400)
万方数据