首页|Fgf8P2A-3×GFP/+:A New Genetic Mouse Model for Specifically Labeling and Sorting Cochlear Inner Hair Cells
Fgf8P2A-3×GFP/+:A New Genetic Mouse Model for Specifically Labeling and Sorting Cochlear Inner Hair Cells
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The cochlear auditory epithelium contains two types of sound receptors,inner hair cells(IHCs)and outer hair cells(OHCs).Mouse models for labelling juvenile and adult IHCs or OHCs exist;however,labelling for embryonic and perinatal IHCs or OHCs are lacking.Here,we gener-ated a new knock-in Fgf8P2A-3×GFP/+(Fgf8GFP/+)strain,in which the expression of a series of three GFP fragments is controlled by endogenous Fgf8 cis-regulatory elements.After confirming that GFP expression accurately reflects the expression of Fgf8,we successfully obtained both embry-onic and neonatal IHCs with high purity,highlighting the power of Fgf8GFP/+.Furthermore,our fate-mapping analy-sis revealed,unexpectedly,that IHCs are also derived from inner ear progenitors expressing Insm1,which is currently regarded as an OHC marker.Thus,besides serving as a highly favorable tool for sorting early IHCs,Fgf8GFP/+ will facilitate the isolation of pure early OHCs by excluding IHCs from the entire hair cell pool.
Fgf8Inner Hair CellInner EarCochlea
Yi Pan、Shuting Li、Shunji He、Guangqin Wang、Chao Li、Zhiyong Liu、Mingliang Xiang
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Department of Otolaryngology and Head and Neck Surgery,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China
Institute of Neuroscience,State Key Laboratory of Neuroscience,CAS Center for Excellence in Brain Science and Intelligence Technology,Chinese Academy of Sciences,Shanghai 200031,China
University of Chinese Academy of Sciences,Beijing 100049,China
Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology,Shanghai 201210,China
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National Key R&D Program of ChinaStrategic Priority Research Program of the Chinese Academy of ScienceNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaShanghai Municipal Science and Technology Major ProjectScience and Technology Commission of Shanghai MunicipalityShanghai Sailing Program
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