The Anterior Insula and its Projection to the Prelimbic Cortex are Involved in the Regulation of 5-HT-Induced Itch

扫码查看

原文链接

万方数据
维普

外文摘要：Itch is an unpleasant sensation that urges people and animals to scratch.Neuroimaging studies on itch have yielded extensive correlations with diverse cortical and sub-cortical regions,including the insular lobe.However,the role and functional specificity of the insular cortex(IC)and its subdivisions in itch mediation remains unclear.Here,we demonstrated by immunohistochemistry and fiber photom-etry tests,that neurons in both the anterior insular cortex(AIC)and the posterior insular cortex(PIC)are activated during acute itch processes.Pharmacogenetic experiments revealed that nonselective inhibition of global AIC neu-rons,or selective inhibition of the activity of glutaminer-gic neurons in the AIC,reduced the scratching behaviors induced by intradermal injection of 5-hydroxytryptamine(5-HT),but not those induced by compound 48/80.How-ever,both nonselective inhibition of global PIC neurons and selective inhibition of glutaminergic neurons in the PIC failed to affect the itching-scratching behaviors induced by either 5-HT or compound 48/80.In addition,pharmacoge-netic inhibition of AIC glutaminergic neurons effectively blocked itch-associated conditioned place aversion behavior,and inhibition of AIC glutaminergic neurons projecting to the prelimbic cortex significantly suppressed 5-HT-evoked scratching.These findings provide preliminary evidence that the AIC is involved,at least partially via aversive emotion mediation,in the regulation of 5-HT-,but not compound 48/80-induced itch.

外文关键词：

ItchInsular cortexPrelimbic cortexConditioned place aversionPharmacogenetic inhibition

作者：

Juan Yao、Xuan Li、Guang-Yan Wu、Bing Wu、Jun-Hui Long、Pu-Jun Wang、Shu-Lei Liu、Jie Gao、Jian-Feng Sui

展开 >

作者单位：

Experimental Center of Basic Medicine,Army Medical University,Chongqing 400038,China

Department of Physiology,College of Basic Medical Sciences,Army Medical University,Chongqing 400038,China

Department of Dermatology,The 958th Army Hospital of the People's Liberation Army,Chongqing 400020,China

State Key Laboratory of Trauma,Burns and Combined Injury,Army Medical Centre of the PLA,Institute of Surgery Research,Daping Hospital,Army Medical University,Chongqing 400042,China

展开 >

基金：

National Natural Science Foundation of ChinaNatural Science Foundation of ChongqingNatural Science Foundation of ChongqingChongqing Medical Scientific Research Project(Joint project of Chongqing Health Commission and Science and Technology Bureau

项目编号：

82271542cstc2020jcyjmsxm X0391cstc2019jcyjmsxm X02692023ZDXM001

出版年：

2023

DOI：

10.1007/s12264-023-01093-y

神经科学通报(英文版)

中国科学院上海生命科学研究院

神经科学通报(英文版)

CSTPCDCSCD

影响因子：0.741

ISSN：1673-7067

年,卷(期)：2023.39(12)

参考文献量59