Abstract
The mammalian brain is a highly complex net-work that consists of millions to billions of densely-inter-connected neurons.Precise dissection of neural circuits at the mesoscopic level can provide important structural infor-mation for understanding the brain.Optical approaches can achieve submicron lateral resolution and achieve"optical sectioning"by a variety of means,which has the natural advantage of allowing the observation of neural circuits at the mesoscopic level.Automated whole-brain optical imag-ing methods based on tissue clearing or histological sec-tioning surpass the limitation of optical imaging depth in biological tissues and can provide delicate structural infor-mation in a large volume of tissues.Combined with various fluorescent labeling techniques,whole-brain optical imaging methods have shown great potential in the brain-wide quan-titative profiling of cells,circuits,and blood vessels.In this review,we summarize the principles and implementations of various whole-brain optical imaging methods and provide some concepts regarding their future development.
基金项目
STI2030-Major Projects(2021ZD0201001)
STI2030-Major Projects(2021ZD0201000)
National Natural Science Foundation of China(81827901)
National Natural Science Foundation of China(32192412)
维普
万方数据