首页|Whole-brain Optical Imaging:A Powerful Tool for Precise Brain Mapping at the Mesoscopic Level

Whole-brain Optical Imaging:A Powerful Tool for Precise Brain Mapping at the Mesoscopic Level

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The mammalian brain is a highly complex net-work that consists of millions to billions of densely-inter-connected neurons.Precise dissection of neural circuits at the mesoscopic level can provide important structural infor-mation for understanding the brain.Optical approaches can achieve submicron lateral resolution and achieve"optical sectioning"by a variety of means,which has the natural advantage of allowing the observation of neural circuits at the mesoscopic level.Automated whole-brain optical imag-ing methods based on tissue clearing or histological sec-tioning surpass the limitation of optical imaging depth in biological tissues and can provide delicate structural infor-mation in a large volume of tissues.Combined with various fluorescent labeling techniques,whole-brain optical imaging methods have shown great potential in the brain-wide quan-titative profiling of cells,circuits,and blood vessels.In this review,we summarize the principles and implementations of various whole-brain optical imaging methods and provide some concepts regarding their future development.

Whole-brain optical imagingOptical sectioningMicrometer resolutionBrain connectomeNeural circuitsNeuron

Tao Jiang、Hui Gong、Jing Yuan

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Huazhong University of Science and Technology-Suzhou Institute for Brainsmatics,Jiangsu Industrial Technology Research Institute,Suzhou 215123,China

Wuhan National Laboratory for Optoelectronics,Huazhong University of Science and Technology,Wuhan 430074,China

STI2030-Major ProjectsSTI2030-Major ProjectsNational Natural Science Foundation of ChinaNational Natural Science Foundation of China

2021ZD02010012021ZD02010008182790132192412

2023

10.1007/s12264-023-01112-y

神经科学通报(英文版)
中国科学院上海生命科学研究院

神经科学通报(英文版)

CSTPCDCSCD
影响因子：0.741
ISSN：1673-7067
年,卷(期)：2023.39(12)
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