One-step cell biomanufacturing platform:porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo

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外文摘要：Numerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson's disease.Transplantation of midbrain dopaminergic progenitor cells is a promising treatment for Parkinson's disease.However,transplanted cells can be injured by mechanical damage during handling and by changes in the transplantation niche.Here,we developed a one-step biomanufacturing platform that uses small-aperture gelatin microcarriers to produce beads carrying midbrain dopaminergic progenitor cells.These beads allow midbrain dopaminergic progenitor cell differentiation and cryopreservation without digestion,effectively maintaining axonal integrity in vitro.Importantly,midbrain dopaminergic progenitor cell bead grafts showed increased survival and only mild immunoreactivity in vivo compared with suspended midbrain dopaminergic progenitor cell grafts.Overall,our findings show that these midbrain dopaminergic progenitor cell beads enhance the effectiveness of neuronal cell transplantation.

外文关键词：

axonal integritycell cryopreservationcellular environmentcellular nichecell replacement therapydopaminergic progenitorshuman pluripotent stem cellmechanical damageneuronal cell deliveryParkinson's diseasesmall-aperture gelatin microcarriers

作者：

Lin Feng、Da Li、Yao Tian、Chengshun Zhao、Yun Sun、Xiaolong Kou、Jun Wu、Liu Wang、Qi Gu、Wei Li、Jie Hao、Baoyang Hu、Yukai Wang

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作者单位：

State Key Laboratory of Stem Cell and Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing,China

Institute for Stem Cell and Regenerative Medicine,Chinese Academy of Sciences,Beijing,China

National Stem Cell Resource Center,Chinese Academy of Sciences,Beijing,China

Savaid Medical School,University of Chinese Academy of Sciences,Beijing,China

Beijing Institute for Stem Cell and Regenerative Medicine,Beijing,China

State Key Laboratory of Membrane Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing,China

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基金：

National Key Research and Development Program of ChinaNational Key Research and Development Program of ChinaNational Key Research and Development Program of ChinaNational Key Research and Development Program of ChinaNational Key Research and Development Program of ChinaNational Key Research and Development Program of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaCAS Project for Young Scientists in Basic ResearchJoint Funds of the National Natural Science Foundation of China

项目编号：

2017YFE01229002019YFA01108002019YFA09038022021YFA11016042018YFA01085022020YFA08040033162100431970821YSBR-041U21A20396

出版年：

2024

DOI：

10.4103/1673-5374.377412

中国神经再生研究(英文版)

中国康复医学会

中国神经再生研究(英文版)

CSTPCD

影响因子：0.902

ISSN：1673-5374

年,卷(期)：2024.19(2)

参考文献量61