首页|Hypoxia-preconditioned bone marrow-derived mesenchymal stem cells protect neurons from cardiac arrest-induced pyroptosis

Hypoxia-preconditioned bone marrow-derived mesenchymal stem cells protect neurons from cardiac arrest-induced pyroptosis

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Cardiac arrest can lead to severe neurological impairment as a result of inflammation,mitochondrial dysfunction,and post-cardiopulmonary resuscitation neurological damage.Hypoxic preconditioning has been shown to improve migration and survival of bone marrow-derived mesenchymal stem cells and reduce pyroptosis after cardiac arrest,but the specific mechanisms by which hypoxia-preconditioned bone marrow-derived mesenchymal stem cells protect against brain injury after cardiac arrest are unknown.To this end,we established an in vitro co-culture model of bone marrow-derived mesenchymal stem cells and oxygen-glucose deprived primary neurons and found that hypoxic preconditioning enhanced the protective effect of bone marrow stromal stem cells against neuronal pyroptosis,possibly through inhibition of the MAPK and nuclear factor κB pathways.Subsequently,we transplanted hypoxia-preconditioned bone marrow-derived mesenchymal stem cells into the lateral ventricle after the return of spontaneous circulation in an 8-minute cardiac arrest rat model induced by asphyxia.The results showed that hypoxia-preconditioned bone marrow-derived mesenchymal stem cells significantly reduced cardiac arrest-induced neuronal pyroptosis,oxidative stress,and mitochondrial damage,whereas knockdown of the liver isoform of phosphofructokinase in bone marrow-derived mesenchymal stem cells inhibited these effects.To conclude,hypoxia-preconditioned bone marrow-derived mesenchymal stem cells offer a promising therapeutic approach for neuronal injury following cardiac arrest,and their beneficial effects are potentially associated with increased expression of the liver isoform of phosphofructokinase following hypoxic preconditioning.

bone marrow-derived mesenchymal stem cellscardiac arrestcardiac resuscitationhypoxic preconditioningliver isoform of phosphofructokinasemitochondrianeuroinflammationoxidative stresspyroptosisreactive oxygen species

Xiahong Tang、Nan Zheng、Qingming Lin、Yan You、Zheng Gong、Yangping Zhuang、Jiali Wu、Yu Wang、Hanlin Huang、Jun Ke、Feng Chen

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Shengli Clinical Medical College of Fujian Medical University,Fujian Medical University,Fuzhou,Fujian Province,China

Department of Emergency,Fujian Provincial Hospital,Fuzhou,Fujian Province,China

Fujian Provincial Key Laboratory of Emergency Medicine,Fuzhou,Fujian Province,China

The Second Department of Intensive Care Unit,Fujian Provincial Hospital South Branch,Fuzhou,Fujian Province,China

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2025

中国神经再生研究(英文版)
中国康复医学会

中国神经再生研究(英文版)

影响因子:0.902
ISSN:1673-5374
年,卷(期):2025.20(4)