首页|Silk-based nerve guidance conduits with macroscopic holes modulate the vascularization of regenerating rat sciatic nerve

Silk-based nerve guidance conduits with macroscopic holes modulate the vascularization of regenerating rat sciatic nerve

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Peripheral nerve injuries induce a severe motor and sensory deficit.Since the availability of autologous nerve transplants for nerve repair is very limited,alternative treatment strategies are sought,including the use of tubular nerve guidance conduits(tNGCs).However,the use of tNGCs results in poor functional recovery and central necrosis of the regenerating tissue,which limits their application to short nerve lesion defects(typically shorter than 3 cm).Given the importance of vascularization in nerve regeneration,we hypothesized that enabling the growth of blood vessels from the surrounding tissue into the regenerating nerve within the tNGC would help eliminate necrotic processes and lead to improved regeneration.In this study,we reported the application of macroscopic holes into the tubular walls of silk-based tNGCs and compared the various features of these improved si l k+tNGCs with the tubes without holes(silk-tNGCs)and autologous nerve transplants in an 8-mm sciatic nerve defect in rats.Using a combination of micro-computed tomography and histological analyses,we were able to prove that the use of silk+tNGCs induced the growth of blood vessels from the adjacent tissue to the intraluminal neovascular formation.A significantly higher number of blood vessels in the silk+group was found compared with autologous nerve transplants and silk-,accompanied by improved axon regeneration at the distal coaptation point compared with the silk-tNGCs at 7 weeks postoperatively.In the 15-mm(critical size)sciatic nerve defect model,we again observed a distinct ingrowth of blood vessels through the tubular walls of si l k+tNGCs,but without improved functional recovery at 12 weeks postoperatively.Our data proves that macroporous tNGCs increase the vascular supply of regenerating nerves and facilitate improved axonal regeneration in a short-defect model but not in a critical-size defect model.This study suggests that further optimization of the macroscopic holes silk+tNGC approach containing macroscopic holes might result in improved grafting technology suitable for future clinical use.

axon regenerationblood vesselfunctional recoverymacroporousnerve lesionperipheral nerve repairsciatic nervesilk-based nerve guidance conduitvascularization

Carina Hromada、Patrick Heimel、Markus Kerbl、László Gál、Sylvia Nürnberger、Barbara Schaedl、James Ferguson、Nicole Swiadek、Xavier Monforte、Johannes C.Heinzel、Antal Nógrádi、Andreas H.Teuschl-Woller、David Hercher

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Department Life Science Engineering,University of Applied Sciences Technikum Wien,Vienna,Austria

Austrian Cluster for Tissue Regeneration,Vienna,Austria

Ludwig Boltzmann Institute for Traumatology,The Research Center in Cooperation with AUVA,Vienna,Austria

University Clinic of Dentistry,Medical University of Vienna,Vienna,Austria

Department of Plastic,Reconstructive and Aesthetic Surgery,Landesklinikum Wiener Neustadt,2700 Wiener Neustadt,Austria

Department of Anatomy,Histology and Embryology,Albert Szent-Györgyi Medical School,University of Szeged,Szeged,Hungary

Medical University of Vienna,Department of Orthopedics and Trauma Surgery,Devision of Trauma Surgery,Vienna,Austria

Department of Hand-,Plastic,Reconstructive and Burn Surgery,BG Unfallklinik Tuebingen,University of Tuebingen,Tuebingen,Germany

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2025

10.4103/NRR.NRR-D-23-01518

中国神经再生研究(英文版)
中国康复医学会

中国神经再生研究(英文版)

影响因子：0.902
ISSN：1673-5374
年,卷(期)：2025.20(6)