Storage time affects the level and diagnostic efficacy of plasma biomarkers for neurodegenerative diseases

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外文摘要：Several promising plasma biomarker proteins,such as amyloid-β(Aβ),tau,neurofilament light chain,and glial fibrillary acidic protein,are widely used for the diagnosis of neurodegenerative diseases.However,little is known about the long-term stability of these biomarker proteins in plasma samples stored at-80℃.We aimed to explore how storage time would affect the diagnostic accuracy of these biomarkers using a large cohort.Plasma samples from 229 cognitively unimpaired individuals,encompassing healthy controls and those experiencing subjective cognitive decline,as well as 99 patients with cognitive impairment,comprising those with mild cognitive impairment and dementia,were acquired from the Sino Longitudinal Study on Cognitive Decline project.These samples were stored at-80℃ for up to 6 years before being used in this study.Our results showed that plasma levels of Aβ42,Aβ40,neurofilament light chain,and glial fibrillary acidic protein were not significantly correlated with sample storage time.However,the level of total tau showed a negative correlation with sample storage time.Notably,in individuals without cognitive impairment,plasma levels of total protein and tau phosphorylated protein threonine 181(p-tau 181)also showed a negative correlation with sample storage time.This was not observed in individuals with cognitive impairment.Consequently,we speculate that the diagnostic accuracy of plasma p-tau181 and the p-tau181 to total tau ratio may be influenced by sample storage time.Therefore,caution is advised when using these plasma biomarkers for the identification of neurodegenerative diseases,such as Alzheimer's disease.Furthermore,in cohort studies,it is important to consider the impact of storage time on the overall results.

外文关键词：

Alzheimer's diseaseamyloid-βdiagnostic abilityglial fibrillary acidic proteinneurodegenerationneurofilament light chainplasma biomarkerssingle molecule arraystorage timetau

作者：

Lifang Zhao、Mingkai Zhang、Qimeng Li、Xuemin Wang、Jie Lu、Ying Han、Yanning Cai

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作者单位：

Department of Clinical Biobank,Xuanwu Hospital,Capital Medical University,Beijing,China

Beijing Geriatric Medical Research Center,Beijing,China

Department of Neurology,Xuanwu Hospital,Capital Medical University,Beijing,China

Department of Radiology and Nuclear Medicine,Xuanwu Hospital,Capital Medical University,Beijing,China

Beijing Key Laboratory of Magnetic Resonance Imaging and Brain Informatics,Beijing,China

Key Laboratory of Neurodegenerative diseases,Ministry of Education,Beijing,China

School of Biomedical Engineering,Hainan University,Haikou,Hainan Province,China

Center of Alzheimer's Disease,Beijing Institute for Brain Disorders,Beijing,China

National Clinical Research Center for Geriatric Diseases,Beijing,China

Institute of Biomedical Engineering,Shenzhen Bay Laboratory,Gaoke Innovation Center,Shenzhen,Guangdong Province,China

Department of Neurobiology,Xuanwu Hospital,Capital Medical University,Beijing,China

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出版年：

2025

DOI：

10.4103/NRR.NRR-D-23-01983

中国神经再生研究(英文版)

中国康复医学会

中国神经再生研究(英文版)

影响因子：0.902

ISSN：1673-5374

年,卷(期)：2025.20(8)