Cloning and expression of Alpha-L-Arabinofuranosidases Gene SpARAF1 from Stipagrostis pennata
Alpha-L-Arabinofuranosidase(ARAF)plays an important role in plant cell wall formation and re-sponse to stress.In order to explore the mechanism and function of gene involved in resistance to abiotic stress and influence on the development of root sand jacket in grass.In this study,SpARAF1 gene was cloned and ana-lyzed by bioinformatics,subcellular localization and real-time fluorescence quantitative PCR.The total length of SpARAF1 coding region is 1 173 bp,encoding 391 amino acids hydrophilic non-transmembrane protein,the rela-tive molecular weight is 43.3 kD.Phylogenetic analysis showed that the protein encoded by this gene belongs to the Alpha-L-AF-C superfamily and is closely related to rice and maize.Subcellular localization showed that SpARAFl was localized in the cell wall.The results of qRT-PCR showed that the expression of SpARAF1 was significantly induced by abiotic stress such as salt,drought and high temperature,which indicated that SpARAF1 was involved in the response process of Stipagrostis pennata to abiotic stress.Protein interaction prediction analy-sis showed that SpARAF1 may interact with carbohydrate kinase and polygalacturonase.
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