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侧流层析技术在大肠杆菌O157∶H7检测中的应用

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大肠杆菌O157∶H7作为一种常见的食源性致病菌,在低感染剂量下即可导致人类患严重疾病.侧流层析技术(LFCA)由于其具有高效分离的特性,能够满足食品中大肠杆菌O157∶H7的快速检测需求.然而,目前广泛应用的LFCA方法,信号强度较弱,检测灵敏度较低,难以实现样本中低浓度大肠杆菌O157∶H7的检出.因此,本文重点整理了近年来出现的新型侧流层析技术,围绕检测效率、灵敏度进行了系统性归纳,比较各方法的优势与短板,为大肠杆菌O157∶H7侧流层析检测技术的发展提供重要结论性指导.
Review on the application of lateral flow chromatographic assay in Escherichia coli O157∶H7 detection
As a common foodborne pathogen,Escherichia coli(E.coli)O157∶H7 can cause serious diseases in human body at low infectious doses.Lateral flow chromatographic assay(LFCA)can meet the requirement of detecting E.coli O157∶H7 in food samples rapidly due to its efficient separation property.At present,the widely-used LFCA methods generate weak signal strength and possess low detection sensitivity,which can hardly detect low concentration of E.coli O157∶H7.Therefore,this review focuses on the newly published LFCA literature,and a systematic induction is performed concerning the detection efficiency and sensitivity of the above studies.The advantages and disadvantages of each method are compared in this review,which will provide important conclusive guidance for the development of LFCA in E.coli O157∶H7 detection.

Lateral flow chromatographic assayfoodborne pathogenicEscherichia coli O157∶H7detectionfood safety

田歌、蒋亦佳、张赟

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新乡医学院医学技术学院,河南新乡 453000

侧流层析技术 食源性致病菌 大肠杆菌O157∶H7 检测 食品安全

河南省科技攻关计划

222102310310

2024

中国食品卫生杂志
中国卫生信息与健康医疗大数据学会

中国食品卫生杂志

CSTPCD北大核心
影响因子:1.173
ISSN:1004-8456
年,卷(期):2024.36(1)
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