上海市售有机和传统面粉中7种真菌毒素的污染状况调查
Investigation on the contamination of organic and conventional wheat flour in Shanghai City with seven kinds of mycotoxins
徐安琪 1秦璐昕 2周海燕 1武爱波 1刘弘 2刘娜1
作者信息
- 1. 中国科学院大学,中国科学院上海营养与健康研究所,上海 200031
- 2. 上海市疾病预防控制中心,上海 200336
- 折叠
摘要
目的 比较上海市售有机和传统面粉中脱氧雪腐镰刀菌烯醇(DON)、玉米赤霉烯酮(ZEN)、伏马毒素B1(FB1)、细交链孢菌酮酸(TeA)、交链孢酚单甲醚(AME)、T-2毒素(T-2)、赫曲霉毒素A(Ochratoxin A,OTA)7种真菌毒素污染情况,并分析面粉中镰刀菌毒素、链格孢毒素和OTA的累积污染情况.方法 80份面粉样品采用乙腈提取,超高效液相色谱-串联质谱法检测采集的面粉样品中7种真菌毒素的含量.7种真菌毒素的线性范围良好,相关系数大于0.99,加标回收率为80.13％～122.86％,RSD为1.91％～17.14％,所建方法简单、快速、准确、精密度高,可用于面粉中多种真菌毒素的检测分析.结果 上海市售的80份面粉中,T-2、FB1无检出,DON、ZEN、AME、OTA的平均值在有机面粉中污染水平高于传统面粉.97.5％的面粉样本中至少两种真菌毒素共存,其中DON+ZEN+OTA共存比例最高,为18.75％.结论 上海市售面粉中,有机面粉中真菌毒素的污染率和污染水平高于传统面粉,面粉中镰刀菌毒素(DON、ZEN)、链格孢毒素(TeA、AME)和赭曲霉毒素A的累积污染值得关注.
Abstract
Objective To compare the levels of contamination of seven mycotoxins,including deoxynivalenol(DON),zearalenone(ZEN),fumonisin B1(FB1),tenuazonic acid(TeA),alternariol monomethvl ether(AME),T-2 Toxin(T-2),ochratoxin A(OTA)in organic and conventional wheat flour sold in Shanghai City,and analyze the cumulative contamination of Fusarium toxin,Alternaria toxin and OTA.Methods Samples were extracted with acetonitrile,and the contents of the seven mycotoxins were detected using ultra-performance liquid chromatography-tandem mass spectrometry.The calibration curves for all mycotoxins were linear,with correlation coefficients above 0.99.The average recoveries of the spiked samples ranged from 80.13％to 122.86％,with relative standard deviations from 1.91％to 17.14％.This method is simple and rapid,with high accuracy and precision,and meets the requirements for mycotoxin analysis in flour.Results T-2 and FB1 were not detected in organic or conventional flours.The contamination levels of DON,ZEN,AME and OTA in organic flour were higher than those in conventional flour.The existence of at least two mycotoxins was observed in 97.5％of the flour samples,with DON+ZEN+OTA having the highest coexistence rate at 18.75％.Conclusion The rate and level of mycotoxin contamination in organic wheat flour were higher than those in conventional wheat flour.The cumulative contamination of Fusarium toxins(DON,ZEN),Alternaria toxins(TeA,AME)and OTA were also observed.
关键词
有机面粉/传统面粉/超高效液相色谱-串联质谱法/真菌毒素/污染水平Key words
Organic flour/conventional flour/ultra performance liquid chromatography-tandem mass spectrometry/mycotoxin/contamination level引用本文复制引用
基金项目
上海市科委技术标准领域项目(20DZ2202600)
出版年
2024
NETL
NSTL
万方数据