植物源食品中菌落总数的测量不确定度评定
Evaluation of the uncertainty in measurement of the aerobic plate count in the plant-derived foods
曲世超 1徐文英 1薛伟锋 1褚莹倩 1齐欣1
作者信息
- 1. 大连海关技术中心,辽宁大连 116000
- 折叠
摘要
目的 对植物源食品中菌落总数的测定结果进行不确定性评定,确保检测结果的准确性.方法 按照食品安全国家标准GB 4789.2-2022的方法,采用平板计数琼脂培养基和测试片分别测定植物源食品中菌落总数,对引入的不确定度分量进行评定,最终分别合成植物源食品在平板计数琼脂培养基和测试片上菌落总数测定的不确定度.结果 在置信概率为95％时,植物源食品在平板计数琼脂培养基和测试片上菌落总数测定结果的扩展不确定度分别为0.045 863和0.049 436,测定结果区间范围分别为7.0×103～1.1×104和7.0×103～1.2×104 CFU/g.结论 植物源食品在平板计数琼脂培养基和测试片上菌落总数测定结果差异不大,针对各自评定数据可对试验样本进行结果判定分析.建议实验室通过提升自身检测能力来降低不确定度分量的引入,为植物源食品的生产、检验和监督等方面提供坚实有力的技术保障.
Abstract
Objective To ensure the accuracy of the results in such products,the uncertainty of the aerobic plate count in the plant-derived foods was evaluated.Methods In accordance with the national standards for food safety GB 4789.2-2022,the aerobic plate count in plant-derived foods was determined by plate count agar medium and test sheets.The introduced uncertainty components in the experiments were evaluated until the synthetic uncertainties of the aerobic plate count in the plant-derived foods on plate count agar medium and test sheets were calculated respectively.Results As the confidence probability was 95％,the extended uncertainties of the aerobic plate count in the plant-derived foods on plate counte agar medium and test sheets were 0.045 863 and 0.049 436,respectively.And the confidence intervals of the measurement result were 7.0×103-1.1×104 and 7.0×103-1.2×104 CFU/g.Conclusion The uncertainties of the aerobic plate count in the plant-derived foods on plate count agar medium and test sheets have no significant difference between them.According to the evaluation data,the results of the test samples can be judged and analyzed.It is suggested to improve in-house detection ability to reduce the introduction of uncertainty.It will provide strong technical support to production,inspection and supervision of the plant-derived foods.
关键词
植物源性食品/菌落总数/不确定度/评定/培养基Key words
Plant-derived foods/aerobic plate count/uncertainty/evaluation/medium引用本文复制引用
基金项目
辽宁省自然科学基金计划面上项目(2023-MS-349)
大连海关科研项目(2022DK04)
出版年
2024
NETL
NSTL
万方数据