实时荧光PCR检测弯曲菌感染病例的粪便和肛拭子样本
Detection of the stool and anal swab samples from outbreaks caused by Campylobacter by real-time PCR
邹林 1贾楠 2张萍 1甄博珺 1郭晓晨 1王芳 1冀国强 2闫爱霞 2康颖 2马红梅 2李颖2
作者信息
- 1. 北京市通州区疾病预防控制中心,北京 101100
- 2. 北京市顺义区疾病预防控制中心,北京 101300
- 折叠
摘要
目的 通过对3起弯曲菌暴发事件中粪便和肛拭子样本采用实时荧光PCR和培养法检测结果的比较,为实验室快速有效地应对此类事件奠定基础.方法 收集3起弯曲菌感染暴发事件中病例的生物样本;使用过滤培养法进行弯曲菌培养检测,分别使用原始样本、增菌24 h和增菌48 h样本提取DNA进行弯曲菌实时荧光PCR检测;使用Kappa检验对实时荧光PCR检测结果和培养法结果进行一致性分析.结果 原始样本、增菌24h和增菌48h实时荧光PCR检测灵敏度分别为90.91％、97.22％和100％,特异度分别为75.00％、84.00％和78.95％,与培养法结果一致性分析的Kappa值分别为0.643、0.813和0.785.结论 实时荧光PCR检测与培养法结合使用是弯曲菌暴发事件处置的有效实验室检测方法.
Abstract
Objective To lay the foundation of quickly and effectively response to such epidemics for laboratories,the detection of the real-time PCR and culture test results of stool and anal swab samples from 3 outbreaks caused by Campylobacter was compared.Methods Biological samples from the 3 outbreaks of Campylobacter were collected.Bacterial culture was performed on the samples using filter culture method.DNA was extracted from the original samples,24 h enriched samples and 48 h enriched samples.Kappa test was used to analyze the results of real-time PCR and culture method.Results The real-time PCR detection sensitivity of original samples,24 h enriched samples and 48 h enriched samples were 90.91％,97.22％and 100％.And the detection specificity were 75.00％,84.00％和78.95％,respectively.Kappa value for the consistency analysis in culture method with real-time PCR based on original samples,24 h enriched culture samples and 48 h enriched samples were 0.643,0.813 and 0.785,respectively.Conclusion The combination of real-time PCR detection and culture detection is an effective laboratory testing method for the respond of Campylobacter outbreaks.
关键词
弯曲菌/荧光定量聚合酶链式反应/培养法/腹泻病例/食源性致病菌Key words
Campylobacter/real-time PCR/bacterial culture/diarrhea cases/foodborne pathogens引用本文复制引用
出版年
2024
NETL
NSTL
万方数据