Detection of the stool and anal swab samples from outbreaks caused by Campylobacter by real-time PCR
Objective To lay the foundation of quickly and effectively response to such epidemics for laboratories,the detection of the real-time PCR and culture test results of stool and anal swab samples from 3 outbreaks caused by Campylobacter was compared.Methods Biological samples from the 3 outbreaks of Campylobacter were collected.Bacterial culture was performed on the samples using filter culture method.DNA was extracted from the original samples,24 h enriched samples and 48 h enriched samples.Kappa test was used to analyze the results of real-time PCR and culture method.Results The real-time PCR detection sensitivity of original samples,24 h enriched samples and 48 h enriched samples were 90.91％,97.22％and 100％.And the detection specificity were 75.00％,84.00％和78.95％,respectively.Kappa value for the consistency analysis in culture method with real-time PCR based on original samples,24 h enriched culture samples and 48 h enriched samples were 0.643,0.813 and 0.785,respectively.Conclusion The combination of real-time PCR detection and culture detection is an effective laboratory testing method for the respond of Campylobacter outbreaks.
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