部分蔬菜及食用菌中氰化物检测方法研究
Determination of cyanide in some vegetables and edible fungi
丁杨 1孙中华 2赵伟3
作者信息
- 1. 哈尔滨市疾病预防控制中心,黑龙江哈尔滨 150056
- 2. 黑龙江省种业技术服务中心,黑龙江哈尔滨 150008
- 3. 黑龙江中医药大学附属第一医院,黑龙江哈尔滨 150040
- 折叠
摘要
目的 建立利用蒸馏-流动注射联用法检测部分蔬菜及食用菌中氰化物的方法.方法 创新前处理方法,利用水蒸气蒸馏技术处理样品有效除掉干扰杂质,再利用连续流动注射仪检测馏出液中氰化物含量.结果 对20份样品进行氰化物检验,蒸馏-流动注射联用法最低检出限为0.0125 mg/kg,最低定量浓度为0.037 5 mg/kg;回收率为97.0％～103.6％;测定结果相对标准偏差RSD(n=6)为3.74％～4.56％.采用异烟酸-吡唑啉酮分光光度法、异烟酸-巴比妥酸分光光度法与此方法进行比对,蒸馏-流动注射联用法检测速度快、数据准确.结论 蒸馏-流动注射联用法检测结果准确可靠,重复性良好,缩短检验时间,适合对大批量蔬菜及食用菌中氰化物快速准确地检测.
Abstract
Objective A method for the detection of cyanide in vegetables and edible fungi by distillation-flow injection method was established.Methods Innovative pretreatment method,the use of water vapor distillation technology to process the sample to effectively remove interfering impurities,and then the use of continuous flow syringe to detect the cyanide content in the distillate.Results 20 samples were tested for cyanide,the detection limit of the method was 0.012 5 mg/kg,the lowest quantitative concentration was 0.037 5 mg/kg;The recovery rate was between 97.0％-103.6％;The relative standard deviation RSD(n=6)was 3.74％-4.56％.The method was compared with isonicotinic acid-pyrazolone Spectrophotometer and isonicotinic acid-barbituric acid Spectrophotometer.Conclusion The results of distillation-flow injection method was accurate and reliable,with good repeatability and the test time was shortened.It was suitable for rapid and accurate detection of cyanide in large quantities of vegetables and edible fungi.
关键词
蔬菜/食用菌/蒸馏-流动注射联用法/氰化物/检测Key words
Vegetables/Edible fungus/Distillation-flow injection method/Cyanide/Detection引用本文复制引用
出版年
2024
NETL
NSTL
万方数据