2014-2019年岳阳市食源性蜡样芽胞杆菌溶血素hblA与磷脂酶C plc基因特征分析
Genetic characterization analysis of hblA and plc genes of foodborne Bacillus cereus in Yueyang City from 2014 to 2019
谢伟岸 1吴日明 2段洁 1陈露 1李小曼 1张风华1
作者信息
- 1. 湖南师范大学生命科学学院,湖南长沙 410081
- 2. 岳阳市疾病预防控制中心,湖南岳阳 414000
- 折叠
摘要
目的 了解湖南省岳阳市2014-2019年食源性蜡样芽胞杆菌菌株病原学特征,并为蜡样芽胞杆菌引起的食物中毒事件的科学防控提供依据.方法 对2014-2019年分离自湖南省岳阳市城区餐饮门店的26株蜡样芽胞杆菌菌株的致病毒力因子溶血素BL的hblA基因和磷脂酶C的plc基因进行序列扩增和测序,并使用Seqman和MEGAX软件对蜡样芽胞杆菌的hblA和plc基因进行遗传进化分析.结果 从岳阳市分离到的蜡样芽胞杆菌hblA、plc毒力基因的同源性与GenBank中的蜡样芽胞杆菌群相比均大于93.0％.结论 岳阳市分离的蜡样芽胞杆菌的hblA和plc基因与GenBank中的蜡样芽胞杆菌群的同源性高,具有一定的亲缘关系.本研究为进一步了解和科学防控蜡样芽胞杆菌引起的食物中毒事件奠定了研究基础.
Abstract
Objective To provide a genetic basis for scientific prevention and control of food poisoning caused by Bacillus cereus,the pathogenic characteristics of foodborne Bacillus cereus strains isolated in Yueyang City Hu'nan Province from 2014 to 2019 were understood.Methods The virulence genes hemolysin A(hblA)and phospholipase C(plc)were sequenced and amplified from 26 Bacillus cereus strains isolated from catering stores in Yueyang City from 2014 to 2019.Seqman and MEGA X software were used to analyze the nucleotide sequences of hblA and plc genes of Bacillus cereus.Results The homology between the coding product of genes hblA and plc of Bacillus cereus isolated from Yueyang City and corresponding Bacillus cereus protein sequences in GenBank were greater than 93.0％.Conclusion The hblA and plc genes of Bacillus cereus isolated from Yueyang City have high homology with Bacillus cereus gene sequences in GenBank,indicating relatively closer genetic relationship.This study lays the research foundation for further understanding and scientific prevention and control of food poisoning events caused by Bacillus cereus.
关键词
蜡样芽胞杆菌/溶血素hblA/磷脂酶C/基因特征/食源性致病菌/湖南省/岳阳市Key words
Bacillus cereus/hemolysin hblA/phospholipase C/genetic characteristics/foodborne pathogenic bacteria/Hunan province/Yueyang City引用本文复制引用
基金项目
湖南省教育厅基金资助项目(2014SK3129)
湖南省教育厅基金资助项目(19C1160)
出版年
2024
NETL
NSTL
万方数据