Objective To develop a rapid and quantitative method for the detection of enrofloxacin in chicken and pork using magnetic solid phase extraction(MSPE)combined with surface enhanced Raman spectroscopy(SERS).Methods Magnetic Fe3O4@covalent organic framework(COF)nanocomposites were synthesized and applied as absorbent to separate and enrich enrofloxacin.The SERS spectra of enrofloxacin were collected by a portable Raman spectrometer with Ag nanoparticles as enhancing substrate.The characteristic SERS signals of enrofloxacin were used to realize its quantification.Results Intensities of SERS signals at 745.77 cm-1 and the logarithmic values of the concentrations of enrofloxacin in the range of 5.0x10-7 to 1.0x10 5 mol/L displayed a good linear relationship with a coefficient of determination of 0.962.The limit of detection and limit of quantitation were 0.07 and 0.23 μg/g respectively.The recoveries of enrofloxacin in chicken and pork were 80.97％～100.98％with relative standard deviations of 1.6％～4.6％.Conclusion The developed method has the advantages of simple in operate,short in time,high in sensitivity and stability,which provides a new method for the rapid detection and on-site detection of enrofloxacin.
Enrofloxacinrapid detectionmagnetic solid phase extractionsurface enhanced Raman spectroscopyantibioticresidue of veterinary drug
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